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- W3031470383 abstract "ObjectiveTo analysis the differences of cytokines and lymphocyte subsets level in peripheral blood between tick-borne encephalitis and healthy controls with cytometric beads array and flow cytometry method. MethodsInpatients due to bitten by ticks from May 2015 to September 2016 in Inner Mongolia Forestry General Hospital with the serum of tick-borne encephalitis virus specific antibody positive and IgM (or IgG ≥1:20) were enrolled in the TBE group; healthy adults in Yakeshi area were enrolled in the control group. The contents of peripheral blood cytokines IL-17A, IFN-γ, TNF-α, IL-10, IL-6, IL-4 and IL-2 in both groups were detected by cytometric beads array. The percentage of peripheral blood T lymphocytes (CD3+, CD3+CD8+, CD3+CD4+), B lymphocytes (CD3- CD19+) and NK cells (CD3- CD16+ CD56+) was detected by flow cytometry. The rank sum test and t test were used to compare the difference of cytokines content and cell percentage between the two groups. ResultsThe levels of 7 cytokines in the peripheral blood of TBE group was higher than those in the control group, and the difference was statistically significant (P mean < 0.05). The percentage of CD3- CD19+ cells in peripheral blood of TBE group was (14.40±6.69), which was higher than that of the control group (10.87±3.26). The difference was statistically significant (t=3.34, P < 0.01). The percentage of other cells in both groups was not statistically significant. ConclusionChanges of IL-17A, IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2 and B cell levels in peripheral blood can play a role in immune imflammatory of tick-borne encephalitis, and can be used as the auxiliary markers for tick-borne encephalitis.Key words: Tick-borne encephalitis; Forest encephalitis; Cytokines; Cytometric bead array" @default.
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- W3031470383 date "2018-02-25" @default.
- W3031470383 modified "2023-09-23" @default.
- W3031470383 title "Detection of Cytokines and Lymphocyte subsets Change in peripheral blood of Tick-borne Encephalitis with Cytometric Bead Array" @default.
- W3031470383 doi "https://doi.org/10.3760/cma.j.issn.1673-4092.2018.01.012" @default.
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