FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3031502936> ?p ?o ?g. }

• W3031502936 endingPage "137" @default.
• W3031502936 startingPage "131" @default.
• W3031502936 abstract "ObjectiveTo investigate the role and possible mechanisms of paracrine in radiation tolerance of non-small cell lung cancer (NSCLC) by observing the effects of radiation-resistant NSCLC H460R cell secretion on the radiosensitivity of parental H460 cells.MethodsH460 cells were inoculated and cultured, and then divided into control group, conditional culturing group, irradiation group and irradiation combined conditional culturing group. One-time irradiation with 137Cs γ-rays was conducted with a dose rate of 1.02 Gy/min. Effect of H460R conditioned media on the radiation sensitivity of H460 cells was observed by clonogenic assay. The percentage of phospho-histone histone H2AX (γH2AX) positive cells was detected by immunofluorescence staining. The cyclical changes of the cells were detected by flow cytometry. The expression of DNA damage-associated proteins γH2AX and Rad51 were detected by Western Blot.ResultsThe results showed that compared with the single γ-rays irradiation, the number of cell clones was significantly increased in the H460R cells after γ-rays irradiation combined with conditional culturing treatment at different doses of 2, 4, and 6 Gy, and the differences were statistically significant (2,4 Gy: all P<0.05; 6 Gy: P<0.01). The results of immunofluorescence staining showed that the percentage of γH2AX positive cells in the conditional culturing group was higher than that in the control group [(39.40±2.51)% vs. (25.21±2.05)%], and the difference was statistically significant (P<0.01). This value in the irradiation combined conditional culturing group was lower than that in irradiated group [(60.48±2.79)% vs. (80.65±2.05)%], and the difference was statistically significant (P<0.001). The flow cytometry analysis showed that the percentage of cells in G2/M phase for the irradiation (4 Gy) combined conditional culturing group was higher than that of irradiated group [(26.83±1.42)% vs. (15.73±1.29)%], and the difference was statistically significant (P<0.001). The Western Blot results showed that γH2AX and Rad51 protein expression in the irradiation (4 Gy) combined conditional culturing group respectively was lower and higher than that in irradiated group.ConclusionIn the tumor microenvironment, radiation-resistant H460R cells can enhance the radioresistance of H460 cells by paracrine, which may be related to the promotion of DNA repair ability after irradiationKey words: Non-small cell lung cancer; Paracrine; Radioresistance; DNA repair" @default.
• W3031502936 created "2020-06-05" @default.
• W3031502936 creator A5003642180 @default.
• W3031502936 creator A5031315906 @default.
• W3031502936 creator A5035520440 @default.
• W3031502936 creator A5056870031 @default.
• W3031502936 creator A5080306619 @default.
• W3031502936 creator A5089341402 @default.
• W3031502936 date "2018-04-28" @default.
• W3031502936 modified "2023-09-23" @default.
• W3031502936 title "Research of radiation resistance delivery through paracrine effects on non-small cell lung cancer" @default.
• W3031502936 doi "https://doi.org/10.3760/cma.j.issn.1673-4181.2018.02.005" @default.
• W3031502936 hasPublicationYear "2018" @default.
• W3031502936 type Work @default.
• W3031502936 sameAs 3031502936 @default.
• W3031502936 citedByCount "0" @default.
• W3031502936 crossrefType "journal-article" @default.
• W3031502936 hasAuthorship W3031502936A5003642180 @default.
• W3031502936 hasAuthorship W3031502936A5031315906 @default.
• W3031502936 hasAuthorship W3031502936A5035520440 @default.
• W3031502936 hasAuthorship W3031502936A5056870031 @default.
• W3031502936 hasAuthorship W3031502936A5080306619 @default.
• W3031502936 hasAuthorship W3031502936A5089341402 @default.
• W3031502936 hasConcept C111337013 @default.
• W3031502936 hasConcept C117262875 @default.
• W3031502936 hasConcept C121332964 @default.
• W3031502936 hasConcept C126322002 @default.
• W3031502936 hasConcept C128240485 @default.
• W3031502936 hasConcept C142724271 @default.
• W3031502936 hasConcept C1491633281 @default.
• W3031502936 hasConcept C153911025 @default.
• W3031502936 hasConcept C159654299 @default.
• W3031502936 hasConcept C16685009 @default.
• W3031502936 hasConcept C170493617 @default.
• W3031502936 hasConcept C185544564 @default.
• W3031502936 hasConcept C203014093 @default.
• W3031502936 hasConcept C206684579 @default.
• W3031502936 hasConcept C26114605 @default.
• W3031502936 hasConcept C2778236600 @default.
• W3031502936 hasConcept C502942594 @default.
• W3031502936 hasConcept C509974204 @default.
• W3031502936 hasConcept C54355233 @default.
• W3031502936 hasConcept C553184892 @default.
• W3031502936 hasConcept C71924100 @default.
• W3031502936 hasConcept C7876069 @default.
• W3031502936 hasConcept C81885089 @default.
• W3031502936 hasConcept C86803240 @default.
• W3031502936 hasConceptScore W3031502936C111337013 @default.
• W3031502936 hasConceptScore W3031502936C117262875 @default.
• W3031502936 hasConceptScore W3031502936C121332964 @default.
• W3031502936 hasConceptScore W3031502936C126322002 @default.
• W3031502936 hasConceptScore W3031502936C128240485 @default.
• W3031502936 hasConceptScore W3031502936C142724271 @default.
• W3031502936 hasConceptScore W3031502936C1491633281 @default.
• W3031502936 hasConceptScore W3031502936C153911025 @default.
• W3031502936 hasConceptScore W3031502936C159654299 @default.
• W3031502936 hasConceptScore W3031502936C16685009 @default.
• W3031502936 hasConceptScore W3031502936C170493617 @default.
• W3031502936 hasConceptScore W3031502936C185544564 @default.
• W3031502936 hasConceptScore W3031502936C203014093 @default.
• W3031502936 hasConceptScore W3031502936C206684579 @default.
• W3031502936 hasConceptScore W3031502936C26114605 @default.
• W3031502936 hasConceptScore W3031502936C2778236600 @default.
• W3031502936 hasConceptScore W3031502936C502942594 @default.
• W3031502936 hasConceptScore W3031502936C509974204 @default.
• W3031502936 hasConceptScore W3031502936C54355233 @default.
• W3031502936 hasConceptScore W3031502936C553184892 @default.
• W3031502936 hasConceptScore W3031502936C71924100 @default.
• W3031502936 hasConceptScore W3031502936C7876069 @default.
• W3031502936 hasConceptScore W3031502936C81885089 @default.
• W3031502936 hasConceptScore W3031502936C86803240 @default.
• W3031502936 hasIssue "2" @default.
• W3031502936 hasLocation W30315029361 @default.
• W3031502936 hasOpenAccess W3031502936 @default.
• W3031502936 hasPrimaryLocation W30315029361 @default.
• W3031502936 hasRelatedWork W1966066869 @default.
• W3031502936 hasRelatedWork W2021083468 @default.
• W3031502936 hasRelatedWork W2085174601 @default.
• W3031502936 hasRelatedWork W2098188801 @default.
• W3031502936 hasRelatedWork W2099972875 @default.
• W3031502936 hasRelatedWork W2102560312 @default.
• W3031502936 hasRelatedWork W2356936953 @default.
• W3031502936 hasRelatedWork W2359317403 @default.
• W3031502936 hasRelatedWork W2371432593 @default.
• W3031502936 hasRelatedWork W2372614292 @default.
• W3031502936 hasRelatedWork W2376075466 @default.
• W3031502936 hasRelatedWork W2378087152 @default.
• W3031502936 hasRelatedWork W2384689173 @default.
• W3031502936 hasRelatedWork W2389657483 @default.
• W3031502936 hasRelatedWork W2392451604 @default.
• W3031502936 hasRelatedWork W2467970118 @default.
• W3031502936 hasRelatedWork W3028850452 @default.
• W3031502936 hasRelatedWork W3030197287 @default.
• W3031502936 hasRelatedWork W3031107049 @default.
• W3031502936 hasRelatedWork W3143652159 @default.
• W3031502936 hasVolume "41" @default.
• W3031502936 isParatext "false" @default.
• W3031502936 isRetracted "false" @default.

• next