Matches in SemOpenAlex for { <https://semopenalex.org/work/W3031509328> ?p ?o ?g. }
- W3031509328 endingPage "6470" @default.
- W3031509328 startingPage "6458" @default.
- W3031509328 abstract "Abstract The dynamic topological structure of telomeric DNA is closely related to its biological function; however, no such structural information on full-length telomeric DNA has been reported due to difficulties synthesizing long double-stranded telomeric DNA. Herein, we developed an EM-PCR and TA cloning-based approach to synthesize long-chain double-stranded tandem repeats of telomeric DNA. Using mechanical manipulation assays based on single-molecule atomic force microscopy, we found that mechanical force can trigger the melting of double-stranded telomeric DNA and the formation of higher-order structures (G-quadruplexes or i-motifs). Our results show that only when both the G-strand and C-strand of double-stranded telomeric DNA form higher-order structures (G-quadruplexes or i-motifs) at the same time (e.g. in the presence of 100 mM KCl under pH 4.7), that the higher-order structure(s) can remain after the external force is removed. The presence of monovalent K+, single-wall carbon nanotubes (SWCNTs), acidic conditions, or short G-rich fragments (∼30 nt) can shift the transition from dsDNA to higher-order structures. Our results provide a new way to regulate the topology of telomeric DNA." @default.
- W3031509328 created "2020-06-05" @default.
- W3031509328 creator A5016130148 @default.
- W3031509328 creator A5020972239 @default.
- W3031509328 creator A5085108258 @default.
- W3031509328 date "2020-06-04" @default.
- W3031509328 modified "2023-10-16" @default.
- W3031509328 title "Dynamic topology of double-stranded telomeric DNA studied by single-molecule manipulation in vitro" @default.
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