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- W3031567175 abstract "Objective Appling co-culture model to observe the effect of hepatic stellate cells (HSC) paracrine pathway on the proliferation and metabolism of adult hepatic progentior cell (AHPC).Methods Establishing HSC and AHPC indirect co-culture model with Transwell six-hole plate.At 24 h and 96 h after co-culture,AHPC clone formation rate was analyzed; 14 days after co-culture,BrdU proliferation assay was done to assess cell proliferation ability.In addition,the cell supernatant was collected for enzyme linked immunosorbent assay (ELISA) to detection of cell metabolism.Results In HSC group,the clone rate of AHPC was averaged (26.88 ± 3.31) ％,compared with the control group was (20.11 ±2.34) ％ (P ＜ 0.05).In control group,BrdU/Albumin double positive cells were (69.50 ± 18.81)/FOV,and HSC group increased 61.29％ (P ＜0.01).In HSC group,the metabolic concentration of Albumin and Urea of the AHPC supernatant were (14.17 ± 3.14) mg/(L· d) and (34.83 ± 11.41) mg/(L· d),increased by 72.17％ (P ＜0.01) and 66.89％ (P ＜0.05),respectively,compared to the control group.Conclusion A certain numbers of HSC would improve clone formation rate,promote the proliferation and cell metabolism function of AHPC.Key words: Hepatic stellate cell; Liver stem cells; Co-culture; Proliferation; Metabolism" @default.
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- W3031567175 date "2014-05-08" @default.
- W3031567175 modified "2023-09-22" @default.
- W3031567175 title "Mouse hepatic stellate cells regulate proliferation and metabolism of adult hepatic progentior cells in vitro" @default.
- W3031567175 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2014.05.023" @default.
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