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- W3031646395 abstract "Objective To prepare the functionalized nano-graphene oxide (nano-GO) particles,and study their targeted fluorescence imaging effects on glioma U251 cells under near infrared (NIR)irradiation.Methods Single-layer nano-GO particles were obtained after ultrasonic oscillation,and then connected with transferrin (Tf) and fluorescein isothiocyanate (FITC) through poly-L-lysine-G3 to prepare functionalized nano-GO-Tf-FITC particles.Sample morphology was observed and the particle sizes were measured under certain transmission electron microscope (TEM).Glioma U251 cells were employed and divided into targeted experimental group (adding functionalized nano-GO-Tf-FITC particles),parallel control group (adding anti-CD71-FITC reagent) and non-targeted experimental group (adding nano-GO-FITC particles).Fluorescence imaging of these groups was determined by fluorescence microscopy.Results Single-layer particles with a range of 20-100 nm were observed under high TEM,and the functionalized nano-GO-Tf-FITC particles were distributed in a pattern of single layer with a diameter smaller than 100 nm.Yellow green color was monitored in the targeted experimental group and parallel control group,and no imaging was monitored in the non-targeted experimental group because of no transferring integration.Conclusion Functionalized nano-GO-Tf-FITC particles are successfully prepared,and show marked targeted imaging effects on glioma U251 cells.Key words: Glioma; Graphene oxide; Transferrin; Nanocrystallization; Fluorescence imaging" @default.
- W3031646395 created "2020-06-05" @default.
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- W3031646395 date "2013-12-15" @default.
- W3031646395 modified "2023-09-27" @default.
- W3031646395 title "Preparation of functionalized nano-graphene oxide particles and their target imaging role in glioma U251 cells" @default.
- W3031646395 doi "https://doi.org/10.3760/cma.j.issn.1671-8925.2013.12.011" @default.
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