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- W3031662783 abstract "Objective To assess the feasibility of using fluorescent dye PKH-26 as a cell tracer in the construction of tissue engineered peripheral nerve.Methods Primary adipose derived stem cells (ADSCs) were isolated by enzymatic digestion of adipose tissue from the back of the neck of New Zealand white rabbits.The ADSCs of passage 3 were labeled with PKH-26 and were observed under fluorescence microscope.Flow cytometry was used to detect the percentage of the labeled cells.The cellular vitality was detected by MTT assay,while the differentiation abilities of the cells were tested by in vitro adipogenic,osteogenic and chondrogenic differentiation.Then the labeled cells were micro-injected into the modified acellular nerve scaffolds to build the tissue engineered nerve.After being cultured in vitro for 1,2 and 4 weeks,scanning electron microscopy and frozen sections were used to observe the survival and migration of the cells in the scaffold.Results PKH-26 dye was effective for labeling ADSCs with a 95.12％ labeling rate.Cell vitality and differentiation abilities were not affected by the PKH-26 dye.Furthermore,ADSCs could survive and migrate in the nerve scaffold.The fluorescence could last for at least 4 weeks.Conclusion PKH-26 can be used as ADSCs label for the construction of tissue engineered peripheral nerve and for short-term cell tracing.Key words: Tissue engineering; Peripheral nerve; ADSCs; Fluorescence tracing; Rabbit" @default.
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- W3031662783 date "2014-02-10" @default.
- W3031662783 modified "2023-10-08" @default.
- W3031662783 title "Application of technique of labeling ADSCs with PKH-26 in tissue engineered peripheral nerve" @default.
- W3031662783 doi "https://doi.org/10.3760/cma.j.issn.1005-054x.2014.01.004" @default.
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