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• W3031683055 abstract "Abstract Two-photon polymerization (TPP) is capable of fabricating 3D structures with dimensions from sub-µm to a few hundred µm. As a direct laser writing (DLW) process, fabrication time of 3D TPP structures scale with the third order, limiting its use in large volume fabrication. Here, we report on a scalable fabrication method that cuts fabrication time to a fraction. A parallelized 9 multi-beamlets DLW process, created by a fixed diffraction optical element (DOE) and subsequent stitching are used to fabricate large periodic high aspect ratio 3D microstructured arrays with sub-micron features spanning several hundred of µm 2 . The wall structure in the array is designed with a minimum of traced lines and is created by a low numerical aperture (NA) microscope objective, leading to self-supporting lines omitting the need for line-hatching. The fabricated periodic arrays are applied in a cell – 3D microstructure interaction study using living HeLa cells. First indications of increased cell proliferation in the presence of 3D microstructures compared to planar surfaces are obtained. Furthermore, the cells adopt an elongated morphology when attached to the 3D microstructured surfaces. Both results constitute promising findings rendering the 3D microstructures a suited tool for cell interaction experiments, e.g. for cell migration, separation or even tissue engineering studies." @default.
• W3031683055 created "2020-06-05" @default.
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• W3031683055 date "2020-05-26" @default.
• W3031683055 modified "2023-10-18" @default.
• W3031683055 title "Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications" @default.
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• W3031683055 doi "https://doi.org/10.1038/s41598-020-64955-9" @default.
• W3031683055 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7250934" @default.
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