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- W3031719030 abstract "ObjectiveTo reveal the prevalence of human metapneumovirus (HMPV) among pediatric patients with acute respiratory infection (ARI).MethodsNasopharyngeal aspirates were collected from hospitalized young children (1-5 years) diagnosed as ARI in Department of Respiratory Disease, Shanxi Provincial Children′s Hospital, Taiyuan city. The nucleoprotein (N) gene of HMPV was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The positive products of PCR were sequenced, made homology and phylogenetic analysis.ResultsOne sample collected in Feb. 2017 and two samples collected in Nov. 2017 were HMPV positive in RT-PCR detection. The homologies of nucleic acid and deduced amino acid sequences of the three positive PCR products with the corresponding HMPV sequences in GenBank were over 83% and 90%, respectively. Phylogenetic analysis revealed that HMPV B1 subgenotype was detected.ConclusionsHMPV B1 subgenotype was the major epidemic HMPV in young children with ARI in Taiyuan city.Key words: Human metapneumovirus; Nucleoprotein; Children; Acute respiratory infection" @default.
- W3031719030 created "2020-06-05" @default.
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- W3031719030 date "2018-06-30" @default.
- W3031719030 modified "2023-09-23" @default.
- W3031719030 title "B1 subgenotype of human metapneumovirus detected in children with acute respiratory infection" @default.
- W3031719030 doi "https://doi.org/10.3760/cma.j.issn.1003-9279.2018.03.006" @default.
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