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- W3031738586 abstract "Objective To evaluate the feasibility of detecting the mutation of IDH1 in glioma patients by high resolution melting(HRM) curve analysis.Methods The gene mutation of IDH1 was detected by HRM method in 9 surgical resection paraffin specimens of glioma,and the result was verified by gene sequencing.Results 6 cases of R132H(CGT ＞ CAT) mutation and 1 case of R132C(CGT ＞ TGT) mutation were found by HRM method.The resluts of direct gene sequencing were consistent with HRM method.Conclusion The HRM method in detecting IDH1 mutation is more efficient and convenient than direct sequencing.Moreover,it has advantages of low-cost and suitable for clinical test.Key words: Glioma; Mutation; IDH1 gene; High resolution melting curve analysis" @default.
- W3031738586 created "2020-06-05" @default.
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- W3031738586 date "2013-11-15" @default.
- W3031738586 modified "2023-09-28" @default.
- W3031738586 title "Application of high resolution melting analysis in detecting IDH1 mutation in glioma patients" @default.
- W3031738586 doi "https://doi.org/10.3760/cma.j.issn.1008-6706.2013.22.009" @default.
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