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- W3031753141 abstract "E. coli β-galactosidase gene (lacZ) and neomycin resistance (neor) gene were introduced into the BN rat acute myelocytic leukemia (BNML) cell line by retrovirus mediated gene transfer. The expression of lacZ and neor gene in the genetically marked leukemia cell lines (LT12nl) was studied by cytochemical X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) staining, DNA analysis and neomycin (G418) resistant colony assay. Different expression patterns of the lacZ gene were found in the clones or LT12nl cell line. The lacZ gene expression seemed to be independent of the G418 selection pressure in majority of the LT12nl cell lines. However, the selection pressure was necessary for maintaining the neomycin resistance in many LT12nl cell lines during long-term in vitro passages.Key words: Acute myelocytic leukemia; gene expression" @default.
- W3031753141 created "2020-06-05" @default.
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- W3031753141 date "1991-12-14" @default.
- W3031753141 modified "2023-09-28" @default.
- W3031753141 title "EXPRESSION OF E. COLI LACZ AND NEOr GENE IN RAT ACUTE MYELOCYTIC LEUKEMIC CELL LINE" @default.
- W3031753141 doi "https://doi.org/10.3760/cma.j.issn.0253-2727.1991.12.104" @default.
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