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- W3031772929 abstract "Objective To study the Effects of Flt-1 gene on the expression of VEGF and proliferation in BT325 cells.Methods The total RNA was extracted from human umbilical vein endothelial cells as the template and Flt-1 gene was amplified by reverse transcription polymerase chain reaction(RT-PCR).By using gene recombination technique,the Flt-1 cDNA was inserted into eukaryotic expression vector pEGFP-C1.The recombinant plasmid was identified by restriction enzyme digestion and sequence analysis.It was infected into BT325 cells.The quantity of VEGF protein in supernatant was detected by ELISA.The proliferation abilities were measured by MTT and FCM.Results The Flt-1 gene could be obtained by RT-PCR.The restriction enzyme digestion and PCR suggested that the recombinant plasmids were constructed successfully.72 hours after transfection,the VEGF protein levels were decreased (transfection group (56.3 ±41.2) pg/ml,untransfection group (95.5 ±35.3) pg/ml(P ＜0.05) and the proliferation rate of cells was inhibited transfection group (42.6 ± 3.7) ％ and untransfection group (92.8 ± 6.6) ％ (P ＜ 0.05).Conclusions Flt-1 gene could inhibit the expression of VEGF and BT325 cells proliferation and induce their apoptosis in vitro.Key words: Flt-1 gene; Gene recombination; BT325 cell line" @default.
- W3031772929 created "2020-06-05" @default.
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- W3031772929 date "2014-09-28" @default.
- W3031772929 modified "2023-09-22" @default.
- W3031772929 title "Effect of Flt-1 gene on the expression of VEGF and proliferation in BT325 cells" @default.
- W3031772929 doi "https://doi.org/10.3760/cma.j.issn.1001-2346.2014.09.024" @default.
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