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- W3031778237 abstract "：Objective To investigategene transfection efficiency in cell culture mediated by chitosan Nanoparticlesimmobilized with Arg-Gly-Asp(arginine-glycine-aspartic acid,RGD)-peptides.MothodsRGD-peptides was immobilized on chitosan by chemical crosslinking. Plasmid pEGFP-Cl wasencapsulated into Chitosan-RGD-nanoparticles (Chitosan-RGD-pEGFP-NPs) by complexcoacervation method. Chitusan-pEGFP-nanoparticles without RGD were used as the control toevaluate gene tranfection efficiency into Hy926 cells in vitro.Results The transfectionefficiency of Chitosan-RGD-pEGFP-NPs is statistically higher thanChitosan-pEGFP-NPs.(35.7% vs 14.3%,P<0.001) Conclusion Chitosan-RGD NPs could act asgene vector to transfect specific gene in vitro and the transfection efficiency is higherthan that of non-RGD chitosan nanoparticles.Key words: Arg-Gly-Asp-peptides; Chitosan; Nanoparticles; Gene vectors" @default.
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- W3031778237 date "2009-06-28" @default.
- W3031778237 modified "2023-10-18" @default.
- W3031778237 title "In vitro study onRGD-immobllized chitosan nanoparticles as gene vectors" @default.
- W3031778237 doi "https://doi.org/10.3760/cma.j.issn.1673-4181.2009.03.001" @default.
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