Matches in SemOpenAlex for { <https://semopenalex.org/work/W3031788802> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W3031788802 endingPage "329" @default.
- W3031788802 startingPage "326" @default.
- W3031788802 abstract "Objective To study the effect of UHRF1 expression inhibition by RNA interference on the radiosensitivity of esophageal cancer cell line TE-1 and its mechanism.Methods Short hairpin RNA (shRNA) targeting UHRF1 gene was introduced into TE-1 cells by lentivector-mediated transfer.The cells were divided into three groups:non-transfected group,negative control (NC)-shRNA-transfected group,and UHRF1-shRNA-transfected group.The mRNA and protein expression levels of UHRF1 in TE-1 cells were measured by RT-PCR and Western blot before and after transfection.After transfection and X-ray radiation,the radiosensitivity of TE-1 cells was evaluated by colony formation assay; the cell cycle and cell apoptosis were determined by flow cytometry; the γ-H2AX (as a marker of DNA damage) level was measured by Western blot.Results After transfection with UHRF1-shRNA,the mRNA and protein expression levels of UHRF1 were significantly decreased in TE-1 cells,as compared with those in the NC-shRNA-transfected group and non-transfected group (0.11 vs 0.96 and 0.98,F =124.21,P =0.000;0.10 vs 0.89 and 0.94,F =125.25,P =0.000).The UHRF1-shRNA-transfected group had sensitization enhancement ratios of 1.53 (D0 ratio) and 1.95 (Dq ratio).X-ray radiation could cause G2/M arrest and increase apoptotic rate and γ-H2AX expression in TE-1 cells.Compared with the two control groups,the UHRF1-shRNA-transfected group showed significantly less G2/M arrest (F =500.15,P =0.000),a significantly higher apoptotic rate (F =100.10,P =0.000),and significantly higher residual γ-H2AX expression (F =61.00,P =0.000) at 24 hours after X-ray radiation.Conclusions RNA interference can effectively inhibit the UHRF1 expression and enhance the radiosensitivity of TE-1 cells.The mechanism may be related to cell cycle regulation,cell apoptosis,and DNA damage repair.Key words: UHRF1 gene ; Radiosensitivity ; G2/M arrest ; Apoptosis ; DNA damage repair" @default.
- W3031788802 created "2020-06-05" @default.
- W3031788802 creator A5001793326 @default.
- W3031788802 creator A5037121594 @default.
- W3031788802 creator A5048032027 @default.
- W3031788802 creator A5066468331 @default.
- W3031788802 creator A5074866697 @default.
- W3031788802 date "2013-07-15" @default.
- W3031788802 modified "2023-10-10" @default.
- W3031788802 title "UHRF1 expression inhibition by RNA interference enhances the radiosensitivity of esophageal cancer cells" @default.
- W3031788802 doi "https://doi.org/10.3760/cma.j.issn.1004-4221.2013.04.019" @default.
- W3031788802 hasPublicationYear "2013" @default.
- W3031788802 type Work @default.
- W3031788802 sameAs 3031788802 @default.
- W3031788802 citedByCount "0" @default.
- W3031788802 crossrefType "journal-article" @default.
- W3031788802 hasAuthorship W3031788802A5001793326 @default.
- W3031788802 hasAuthorship W3031788802A5037121594 @default.
- W3031788802 hasAuthorship W3031788802A5048032027 @default.
- W3031788802 hasAuthorship W3031788802A5066468331 @default.
- W3031788802 hasAuthorship W3031788802A5074866697 @default.
- W3031788802 hasConcept C104317684 @default.
- W3031788802 hasConcept C126322002 @default.
- W3031788802 hasConcept C153911025 @default.
- W3031788802 hasConcept C166703698 @default.
- W3031788802 hasConcept C173396325 @default.
- W3031788802 hasConcept C190232843 @default.
- W3031788802 hasConcept C190283241 @default.
- W3031788802 hasConcept C206684579 @default.
- W3031788802 hasConcept C2776415932 @default.
- W3031788802 hasConcept C29537977 @default.
- W3031788802 hasConcept C509974204 @default.
- W3031788802 hasConcept C54009773 @default.
- W3031788802 hasConcept C54355233 @default.
- W3031788802 hasConcept C55493867 @default.
- W3031788802 hasConcept C67705224 @default.
- W3031788802 hasConcept C71924100 @default.
- W3031788802 hasConcept C81885089 @default.
- W3031788802 hasConcept C86803240 @default.
- W3031788802 hasConceptScore W3031788802C104317684 @default.
- W3031788802 hasConceptScore W3031788802C126322002 @default.
- W3031788802 hasConceptScore W3031788802C153911025 @default.
- W3031788802 hasConceptScore W3031788802C166703698 @default.
- W3031788802 hasConceptScore W3031788802C173396325 @default.
- W3031788802 hasConceptScore W3031788802C190232843 @default.
- W3031788802 hasConceptScore W3031788802C190283241 @default.
- W3031788802 hasConceptScore W3031788802C206684579 @default.
- W3031788802 hasConceptScore W3031788802C2776415932 @default.
- W3031788802 hasConceptScore W3031788802C29537977 @default.
- W3031788802 hasConceptScore W3031788802C509974204 @default.
- W3031788802 hasConceptScore W3031788802C54009773 @default.
- W3031788802 hasConceptScore W3031788802C54355233 @default.
- W3031788802 hasConceptScore W3031788802C55493867 @default.
- W3031788802 hasConceptScore W3031788802C67705224 @default.
- W3031788802 hasConceptScore W3031788802C71924100 @default.
- W3031788802 hasConceptScore W3031788802C81885089 @default.
- W3031788802 hasConceptScore W3031788802C86803240 @default.
- W3031788802 hasIssue "04" @default.
- W3031788802 hasLocation W30317888021 @default.
- W3031788802 hasOpenAccess W3031788802 @default.
- W3031788802 hasPrimaryLocation W30317888021 @default.
- W3031788802 hasRelatedWork W2137337853 @default.
- W3031788802 hasRelatedWork W2347967057 @default.
- W3031788802 hasRelatedWork W2356260193 @default.
- W3031788802 hasRelatedWork W2371524943 @default.
- W3031788802 hasRelatedWork W2372518804 @default.
- W3031788802 hasRelatedWork W2377429674 @default.
- W3031788802 hasRelatedWork W2384290802 @default.
- W3031788802 hasRelatedWork W2386672376 @default.
- W3031788802 hasRelatedWork W2397792236 @default.
- W3031788802 hasRelatedWork W3027352684 @default.
- W3031788802 hasRelatedWork W3028883206 @default.
- W3031788802 hasRelatedWork W3030615168 @default.
- W3031788802 hasRelatedWork W3030671088 @default.
- W3031788802 hasRelatedWork W3031149958 @default.
- W3031788802 hasRelatedWork W3031783979 @default.
- W3031788802 hasRelatedWork W3032014490 @default.
- W3031788802 hasRelatedWork W3032690840 @default.
- W3031788802 hasRelatedWork W69848800 @default.
- W3031788802 hasRelatedWork W2738303113 @default.
- W3031788802 hasRelatedWork W339818269 @default.
- W3031788802 hasVolume "22" @default.
- W3031788802 isParatext "false" @default.
- W3031788802 isRetracted "false" @default.
- W3031788802 magId "3031788802" @default.
- W3031788802 workType "article" @default.