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- W3031794135 abstract "目的:观察兔自体骨髓基质细胞(marrow stromal cells,BMSCs)在玻璃体腔内的存活情况.方法:采用贴壁筛选法将兔骨髓进行体外培养以获得大量扩增的骨髓基质细胞,移植前将骨髓基质细胞用DAPI标记后注射入自体玻璃体腔内.移植4w后处死动物,取眼球做连续冷冻切片,进行HE染色和荧光显微镜检查.结果:玻璃体腔内可见到DAP1阳性细胞.连续切片HE染色显示玻璃体腔内细胞聚集成团,细胞形状不规则,伸出突起,胞核大而圆,类似视网膜外颗粒层样细胞.结论:移植BMSCs在玻璃体腔内至少能存活4w,在眼内微环境中有向视网膜样细胞分化的趋势.玻璃体内移植是一种简便、可行的移植方法。" @default.
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- W3031794135 date "2004-03-01" @default.
- W3031794135 modified "2023-09-25" @default.
- W3031794135 title "Investigation of rabbit bone marrow stromal cells autotransplanted into the vitreous cavity" @default.
- W3031794135 doi "https://doi.org/10.3760/cma.j.issn.1008-1801.2004.01.016" @default.
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