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- W3031975244 abstract "Objective A study of using interleukin (IL)-ⅠRa and transforming growth factor-β1 (TGF-β1) transfect knee articular chondrocytes of the rabbit in vitro respectively and commonly,and observing expression of IL-ⅠRa and TGF-31 in the knee articular chondrocytes of the rabbit.Methods To use expressed cartier PLNCX2-IL-1Ra-GFP and PLNCX2-TGF-β1-RFP with aim gene transfection the knee articular chondrocytes of the rabbit respectively and commonly,to detect the effect of the gene transfection on articular chondrocytes by NO-detecting kit and the expression of hIL-ⅠRa and hTGF-β1 in cell culture supemate by Elisa method.Results Enzyme digestion identification showed that PLNCX2-IL-1Ra-GFP and PLNCX2-TGF-β1-RFP were constructed successfully.After transfecting articular chondrocytes,using immunofluorescence inverted microscope can detect the green fluorescence in the TGF-β1 group,the red fluorescence in the IL-1Ra group and the green and red fluorescence in the same cell of the cotransfected group,which tested the transfection are successful.In the culture medium,the NO content-test shows that transfected group [hlL-1Ra:(92.15 ±5.36) μmol/L;hTGF-β1:(89.71 ±5.43) μmol/L;hIL-1Ra +hTGF-β1:(94.93 ±4.88) μmol/L] is more than non-transfected group (60.19 ±4.68),(57.23 ±4.29) μmol/L and there was no statistical significance among transfected groups (P ＞ 0.05) and nontransfected group (P ＞ 0.05),there was statistical significance between transfected group and non-transfected group (P ＜0.05).Detecting expression of hIL-1 Ra and hTGF-β1 is found in the transfected groupby enzyme linked immunosorbent assay (ELISA),there was no expression in the non-transfected group and the nude carrier group.There was statistical significance between the transfected group and the non-transfected group (P ＜ 0.05),no statistical significance between the single gene group and the cotransfected gene group (P ＞ 0.05).Conclusion IL-1 Ra and TGF-β1 mediated by retrovirus PLNCX2 can successfully transfect knee articular chondrocytes of the rabbit respectively and commonly and get stably expression,meanwhile the transfected cell have an active life,which provide IL-1 Ra and TGF-β1 respectively and commonly treating OA with theoretical basis.Key words: Retrovirus; Interleukin-1 Ra; Transforming growth tactor-β1 ; Gene transfection ; Chondrocytes" @default.
- W3031975244 created "2020-06-05" @default.
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- W3031975244 date "2013-01-08" @default.
- W3031975244 modified "2023-10-11" @default.
- W3031975244 title "In vitro transfection of interleukin-1Ra and transforming growth factor-β1 mediated with retrovirus PLNCX2 into knee articular chondocytes of rabbits" @default.
- W3031975244 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2013.01.041" @default.
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