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- W3031987025 abstract "Objective To construct and identify recombinant adenovirus expressing prodynorphin gene (PDP) in vector Ad5 F35.Methods Restriction fragment of PDP from pUC57-PDP was inserted in the vector of pDC316.The insertion and the direction of the insert were confirmed by polymerase chain reaction (PCR) and restriction enzyme digestion.The constructed pDC316-PDP was co-transfected with adenovirus backbone pBHG-fiberS/35 into 293 cells to establish the recombinant adenovims Ad5F35-PDP, which was confirmed by PCR.Results The gene sequence coding PDP was identical with that of GenBank by DNA sequencing, and the construction of pDC316-PDP was completed.The significant virus plaques was observed in the 293 cells co-transfected with pDC316-PDP and pBHG-fiber5/35, indicating the successful homologue recombination and virus packaging of the interest fragment in 293 cells.The successful construction of the recombinant adenovirus Ad5F35-PDP with PCR was confirmed, and the titer of Ad5F35-PDP was approximately 1 × 1012 v.p./ml.Conclusion The high-titer,purified recombinant adenovirus of Ad5F35-PDP which contains the full-length coding region of PDP has been established, which could be used in the gene therapy for morphine addiction, and to test the bio-safety and biological function of Ad5F35-PDP in the future.Key words: Dynorphin; Recombinant adenovirus; Gene therapy" @default.
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- W3031987025 date "2009-08-08" @default.
- W3031987025 modified "2023-09-28" @default.
- W3031987025 title "Construction and identification of recombinant adenovirus Ad5F35 containing prodynorphin gene" @default.
- W3031987025 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2009.08.045" @default.
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