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- W3032023248 abstract "目的 在大肠埃希菌中表达磷脂氢谷胱甘肽过氧化物酶(phospholipid hydroperoxide glutathioneperoxidase,PHGPx),以期用于抗PHGPx抗体的制备.方法用特异引物从基因文库中扩增出PHGPxcDNA,与表达载体重组,转化大肠埃希菌.重组体中的PHGPx经点突变作用,编码硒半胱氨酸的密码子UGA变为编码半胱氨酸的UGU.结果序列分析表明,克隆载体中包含PHGPx的完整编码序列及3'未翻译区序列;经SDS-PAGE及Western Blot证实PHGPx在大肠埃希菌中获得了成功表达.结论突变的PGPGx可以在大肠埃希菌中高效表达并可用于制备抗PHGPx抗体。" @default.
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- W3032023248 date "2004-03-20" @default.
- W3032023248 modified "2023-09-25" @default.
- W3032023248 title "Cloning and expression of human phospholipid hydroperoxide glutathione peroxidase in Escherichia coli" @default.
- W3032023248 doi "https://doi.org/10.3760/cma.j.issn.1000-4955.2004.02.008" @default.
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