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- W3032027610 abstract "目的研究HLA-G1基因抑制人自然杀伤细胞(NK细胞)对猪血管内皮细胞杀伤的作用.方法利用脂质体介导的基因转染技术将pcDNA3-HLA-G1转入原代培养的猪血管内皮细胞,以间接免疫荧光技术在蛋白质水平上检测HLA-G1分子在猪内皮细胞上的表达;以NK细胞系(NK92)和外周血单个核细胞为效应细胞,用四甲基偶氮唑盐(MTT)法检测转染细胞对NK细胞杀伤活性的抑制效应.结果与未转染HLA-G1的对照组相比,NK92和外周血单个核细胞对转有HLA-G1的猪血管内皮细胞的杀伤效率均有明显降低(P＜ 0.05).结论 HLA-G1分子可以明显抑制人NK细胞对猪血管内皮细胞的杀伤." @default.
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- W3032027610 date "2003-09-20" @default.
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- W3032027610 title "A study of HLA-G1 protecting porcine endothelial cells against human NK cytotoxicity" @default.
- W3032027610 doi "https://doi.org/10.3760/cma.j.issn.0254-1785.2003.05.009" @default.
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