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• W3032035780 abstract "Rapid monitoring of biological particulate matter (Bio-PM, bioaerosols) requires an enrichment technique for concentrating the Bio-PM dispersed in the air into a small volume of liquid. In this study, an electrostatic air sampler is employed to capture aerosolized test bacteria in a carrier liquid (aerosol-to-hydrosol (ATH) enrichment). Simultaneously, the captured bacteria are carried into a fluid channel for hydrosol-to-hydrosol (HTH) enrichment with Concanavalin A coated magnetic particles (CMPs). The ATH enrichment capacity of the air sampler was evaluated with an aerosol particle counter for the following test bacteria: Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Acinetobacter baumannii. Then, the HTH enrichment capacity for the ATH-collected sample was evaluated using the colony-counting method, scanning electron microscopy based image analysis, fluorescence microscopy, electrical current measurements, and real-time quantitative polymerase chain reaction (qPCR). The ATH and HTH enrichment capacities for the given operation conditions were up to 80 000 and 14.9, respectively, resulting in a total enrichment capacity of up to 1.192 × 106. Given that air-to-liquid enrichment required to prepare detectable bacterial samples for real-time qPCR in field environments is of the order of at least 106, our method can be used to prepare a detectable sample from low-concentration airborne bacteria in the field and significantly reduce the time required for Bio-PM monitoring because of its enrichment capacity." @default.
• W3032035780 created "2020-06-05" @default.
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• W3032035780 date "2020-06-04" @default.
• W3032035780 modified "2023-10-16" @default.
• W3032035780 title "Aerosol-to-Hydrosol Sampling and Simultaneous Enrichment of Airborne Bacteria For Rapid Biosensing" @default.
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• W3032035780 doi "https://doi.org/10.1021/acssensors.0c00555" @default.
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