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- W3032079423 abstract "目的 探索小鼠骨髓单个核细胞(BM MNCs)体外诱导分化为肝细胞的可行性及方法.方法 将四氯化碳注射的小鼠肝脏进行培养,收集上清液,用于诱导BM MNCs分化.观察细胞分化中的形态变化;在1、7、14、21 d时,逆转录聚合酶链反应(RT-PCR)和免疫荧光反应检测甲胎蛋白(AFP)、细胞角蛋白18(CK18)、CK19、肝细胞核因子3β(HNF3β)、酪胺酸胺基转移酶(TAT)和白蛋白(ALB)基因的表达,过碘酸Schiff氏剂反应(PAS)检测细胞糖原合成.结果 诱导组细胞呈肝细胞样变化;RT-PCR检测,第7天可见AFP、CK19基因表达,第14天AFP表达增强,第21天减弱;第14天可见ALB、CK18、HNF3β基因表达,且持续到第21天;第21天TAT表达.免疫荧光反应结果显示,第21天ALB、CK18、CK19、AFP表达呈阳性;同时PAS糖原合成反应也出现阳性.结论 BMMNCs在损伤肝脏条件培养液诱导下可跨越分化为肝细胞,该方法的建立对分化机制研究有重要意义。" @default.
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- W3032079423 date "2006-07-26" @default.
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- W3032079423 title "Injured liver conditional culture medium induces differentiation of adult rat mesenchymal stem cells into ahepatocyte lineage in vitro" @default.
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