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- W3032083637 abstract "Objective To construct a recombinant eukaryotic expression vector encoding rat COX-2 antisense RNA and investigate its effects on rat liver cancer cell proliferatiion. Methods The plasmid encoding anti-sense COX-2 was constructed by using cloning COX-2 cDNA fragment in the reverse direction into the pcDNA3. 1. Then the plasmid pcDNA3. l/COX-2as was transfered into rat hepatocacinoma cell line CBRH7919 with liposome and homologous recombination cell was named as CBRH7919-A. The cell proliferation, cell cycle and apoptosis were analyzed by MTT, flow cytometry and RT-PCR. CBRH7919 cells with or without pretreatment were inoculated into nude mice and the cell proliferation was observed in vivo. Results CBRH7919 treated with antisense COX-2 greatly inhibited the proliferation with a rate of 78% and DNA synthesis (PI of COX-2 group vs control group, 0. 361 vs 0. 784) of CBRH7919 cell line, decreased coloning formation in anchorage-independent assay. In vivo tumorigenic rate was greatly reduced in athymic nude mice as compared with untreated cell group (25% vs 100%). Apoptosis-related gene expression was not different as compared with untreated cell group (P>0. 05). Conclusion Antisense COX-2 RNA can inhibit the proliferation of rat liver cancer cell line in vitro as well as in vivo, suggesting that it has potential value in hepatocellular cancer gene therapy.Key words: Carcinoma hepatocellular; Liposome; Rat; Cyclooxygenase-2; Antisense RNA" @default.
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- W3032083637 date "2009-12-28" @default.
- W3032083637 modified "2023-09-23" @default.
- W3032083637 title "Effects of liposome-mediated rat cyclooxygenase-2 antisense RNA on growth of hepatocarcinoma cells" @default.
- W3032083637 doi "https://doi.org/10.3760/cma.j.issn.1007-8118.2009.12.011" @default.
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