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- W3032141102 abstract "目的通过对人脐血中CD4+和CD8+ T淋巴细胞经特异抗原刺激后,胞内细胞因子γ-IFN和IL-4分泌水平的研究,探讨人脐血干细胞移植后急、慢性移植物抗宿主病(GVHD)发生低下的可能机制.方法 15份脐血和15份健康成人外周血中T淋巴细胞在莫能霉素存在的情况下,体外经十四烷酰拂波醇乙脂和离子霉素刺激后,分别进行CD4-FITC、CD8-FITC荧光单抗染色和γ-IFN-PE、IL-4-PE荧光单抗胞内染色,最后进行流式细胞仪分析.结果脐血中CD4+ Th细胞和CD8+ Tc细胞胞内γ-IFN分泌水平均明显低于成人外周血中CD4+和CD8+T细胞,而脐血CD8+ T细胞胞内IL-4分泌水平与成人外周同类细胞差异无显著性.结论人脐血中T淋巴细胞受特异抗原刺激后,胞内不能产生正常的Th1/Tc1样细胞因子谱,即活化的脐血T淋巴细胞Th1/Tc1样反应低下,这可能是脐血移植后GVHD发生低下的原因之一." @default.
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- W3032141102 date "2001-11-20" @default.
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- W3032141102 title "Study on the intracellular cytokine profile in the activated human umbilical cord blood T lymphocytes" @default.
- W3032141102 doi "https://doi.org/10.3760/cma.j.issn.0254-1785.2001.06.009" @default.
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