FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3032151504> ?p ?o ?g. }

• W3032151504 endingPage "544" @default.
• W3032151504 startingPage "535" @default.
• W3032151504 abstract "Expanding and reprogramming the genetic code of cells for the incorporation of multiple distinct non-canonical amino acids (ncAAs), and the encoded biosynthesis of non-canonical biopolymers, requires the discovery of multiple orthogonal aminoacyl–transfer RNA synthetase/tRNA pairs. These pairs must be orthogonal to both the host synthetases and tRNAs and to each other. Pyrrolysyl–tRNA synthetase (PylRS)/PyltRNA pairs are the most widely used system for genetic code expansion. Here, we reveal that the sequences of ΔNPylRS/ΔNPyltRNA pairs (which lack N-terminal domains) form two distinct classes. We show that the measured specificities of the ΔNPylRSs and ΔNPyltRNAs correlate with sequence-based clustering, and most ΔNPylRSs preferentially function with ΔNPyltRNAs from their class. We then identify 18 mutually orthogonal pairs from the 88 ΔNPylRS/ΔNPyltRNA combinations tested. Moreover, we generate a set of 12 triply orthogonal pairs, each composed of three new PylRS/PyltRNA pairs. Finally, we diverge the ncAA specificity and decoding properties of each pair, within a triply orthogonal set, and direct the incorporation of three distinct non-canonical amino acids into a single polypeptide. Non-canonical amino acids (ncAAs) can be incorporated into proteins in cells using orthogonal aminaocyl–tRNA synthetase/tRNA pairs; the most widely adopted system is based on a pyrrolysyl–tRNA synthetase (PylRS)/tRNA pair. Now, three new PylRS/tRNA pairs have been developed that are mutually orthogonal and can be used together to site-specifically incorporate three distinct ncAAs into a single protein." @default.
• W3032151504 created "2020-06-05" @default.
• W3032151504 creator A5013562042 @default.
• W3032151504 creator A5015228689 @default.
• W3032151504 creator A5077915220 @default.
• W3032151504 creator A5085495473 @default.
• W3032151504 date "2020-05-29" @default.
• W3032151504 modified "2023-10-10" @default.
• W3032151504 title "Engineered triply orthogonal pyrrolysyl–tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids" @default.
• W3032151504 cites W1607154936 @default.
• W3032151504 cites W1965016057 @default.
• W3032151504 cites W1966087112 @default.
• W3032151504 cites W1971372184 @default.
• W3032151504 cites W1973268713 @default.
• W3032151504 cites W1992502609 @default.
• W3032151504 cites W1998014371 @default.
• W3032151504 cites W2002336386 @default.
• W3032151504 cites W2025994431 @default.
• W3032151504 cites W2034933336 @default.
• W3032151504 cites W2039746015 @default.
• W3032151504 cites W2042409375 @default.
• W3032151504 cites W2047360542 @default.
• W3032151504 cites W2052858356 @default.
• W3032151504 cites W2059253719 @default.
• W3032151504 cites W2071639287 @default.
• W3032151504 cites W2076992251 @default.
• W3032151504 cites W2081262930 @default.
• W3032151504 cites W2084922131 @default.
• W3032151504 cites W2090855681 @default.
• W3032151504 cites W2100631905 @default.
• W3032151504 cites W2106687445 @default.
• W3032151504 cites W2111158627 @default.
• W3032151504 cites W2123467717 @default.
• W3032151504 cites W2133450786 @default.
• W3032151504 cites W2135621733 @default.
• W3032151504 cites W2142189265 @default.
• W3032151504 cites W2148123458 @default.
• W3032151504 cites W2157680389 @default.
• W3032151504 cites W2160663329 @default.
• W3032151504 cites W2336207123 @default.
• W3032151504 cites W2400520117 @default.
• W3032151504 cites W2533711759 @default.
• W3032151504 cites W2560707317 @default.
• W3032151504 cites W2617704854 @default.
• W3032151504 cites W2763278454 @default.
• W3032151504 cites W2767046452 @default.
• W3032151504 cites W2768867213 @default.
• W3032151504 cites W2777700910 @default.
• W3032151504 cites W2803722031 @default.
• W3032151504 cites W2883738486 @default.
• W3032151504 cites W2894236370 @default.
• W3032151504 cites W2902321532 @default.
• W3032151504 cites W2918063357 @default.
• W3032151504 cites W2923401475 @default.
• W3032151504 cites W2944969418 @default.
• W3032151504 cites W2952214797 @default.
• W3032151504 cites W3136918052 @default.
• W3032151504 cites W89691187 @default.
• W3032151504 doi "https://doi.org/10.1038/s41557-020-0472-x" @default.
• W3032151504 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7116526" @default.
• W3032151504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32472101" @default.
• W3032151504 hasPublicationYear "2020" @default.
• W3032151504 type Work @default.
• W3032151504 sameAs 3032151504 @default.
• W3032151504 citedByCount "85" @default.
• W3032151504 countsByYear W30321515042020 @default.
• W3032151504 countsByYear W30321515042021 @default.
• W3032151504 countsByYear W30321515042022 @default.
• W3032151504 countsByYear W30321515042023 @default.
• W3032151504 crossrefType "journal-article" @default.
• W3032151504 hasAuthorship W3032151504A5013562042 @default.
• W3032151504 hasAuthorship W3032151504A5015228689 @default.
• W3032151504 hasAuthorship W3032151504A5077915220 @default.
• W3032151504 hasAuthorship W3032151504A5085495473 @default.
• W3032151504 hasBestOaLocation W30321515042 @default.
• W3032151504 hasConcept C104317684 @default.
• W3032151504 hasConcept C10853874 @default.
• W3032151504 hasConcept C125411270 @default.
• W3032151504 hasConcept C153957851 @default.
• W3032151504 hasConcept C169760540 @default.
• W3032151504 hasConcept C185592680 @default.
• W3032151504 hasConcept C2779315393 @default.
• W3032151504 hasConcept C3018844941 @default.
• W3032151504 hasConcept C515207424 @default.
• W3032151504 hasConcept C54355233 @default.
• W3032151504 hasConcept C55493867 @default.
• W3032151504 hasConcept C67705224 @default.
• W3032151504 hasConcept C71240020 @default.
• W3032151504 hasConcept C86803240 @default.
• W3032151504 hasConcept C95444343 @default.
• W3032151504 hasConceptScore W3032151504C104317684 @default.
• W3032151504 hasConceptScore W3032151504C10853874 @default.
• W3032151504 hasConceptScore W3032151504C125411270 @default.
• W3032151504 hasConceptScore W3032151504C153957851 @default.
• W3032151504 hasConceptScore W3032151504C169760540 @default.
• W3032151504 hasConceptScore W3032151504C185592680 @default.
• W3032151504 hasConceptScore W3032151504C2779315393 @default.
• W3032151504 hasConceptScore W3032151504C3018844941 @default.

• next