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- W3032159512 abstract "Objective To investigate the effects of silencing Sirt3 on hypoxia-inducible factor-1α(HIF-1α) and tight junctions (TJs) of Caco-2 cells.Methods The Caco-2 cells were cultured in vitro,and then divided into 4 groups:the normoxia group (Nx),the normoxia plus Sirt3-siRNA group,the hypoxia group (Hx,1％ O2 for 6 h),and the hypoxia plus Sirt3-siRNA group.Transepithelial electrical resistance (TER) was determined.The protein expression of Sirt3 and HIF-1α was detected by using Western blotting.Occludin,Claudin-1 and zonulaoccludens-1 (ZO-1) expression was examined by using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting.Results Sirt3 protein expression was significantly reduced (52％,35％) after transfection of Sirt3-siRNA.The expression levels of HIF-1α protein (0.93 ± 0.01) was increased in the hypoxia plus Sirt3-siRNA group as compared with those under hypoxia for 6 h and normoxia.The expression of TJs mRNA (ZO-1:0.88 ±0.03,Occludin:0.80±0.01,and Claudin-1:0.84±0.01) (P＜0.01),andTJS protein (ZO-1:0.72 ±0.02,Occludin:0.71 ±0.03,Claudin-1:0.69 ±0.01) (P ＜0.01) were decreased after Sirt3-siRNA transfection as compared with that before transfection.The TER value in the hypoxia plus Sirt3-siRNA group was decreased by 30.8％ as compared with hypoxia for 6 h.Conclusion Sirt3 could regulate the expression of HIF-1α and TJs under hypoxia,and then influence the epithelial barrier function.Key words: Epithelial barrier function; RNA interference; Deacetylase Sirt3 ; Hypoxia inducible transcription factor 1 ; Tight junctions" @default.
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- W3032159512 date "2014-02-08" @default.
- W3032159512 modified "2023-10-17" @default.
- W3032159512 title "Effects of silencing deacetylase Sift3 on hypoxia inducible factor-1α and epithelial barrier function of Caco-2 cells under hypoxia" @default.
- W3032159512 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2014.02.021" @default.
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