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- W3032162007 abstract "ObjectiveTo study the proliferation, collagen production and related gene expression in keloids and normal skin fibroblast.MethodsIsolated primary cells of keloid fibroblasts (KFb, n=12) and normal human dermal fibroblasts (NFb, n=12) were identified, the cell viability and proliferating potential and the cell cycle were detected, and the difference on the collagen synthesis between KFb and NFb were compared. The expression of cell cycle-associated genes such as p21, p16, and p27 was dectected by real-time fluorescent quantitative PCR.ResultsThe phase contrast optical microscopy imaging showed that both KFb isolated from keloid tissues and NFb from normal skin tissues possessed classic and similar fibroblast morphology. But there was a significant difference between cell proliferation, Hyp [(2.30±0.10) μg/ml vs. (1.66±0.13) μg/ml, P<0.05] and collagen levels [(17.19±0.75) μg/ml vs. (12.37±0.94) μg/ml, P<0.05]. Compared with NFb, KFb exhibited more percentage of G2/M phase cells [(5.90±0.62)% vs. (16.94 %±1.93)%, P<0.05] and less percentage of G0/G1 phase cells [(90.24 ±2.27)% vs. (75.65±1.92)%, P<0.05]. Cell cycle related genes p16, p21 and p27 were low expressed. Collagen typeⅠwas highly expressed at mRNA levels in KFb than that in NFb [0.84±0.11, 1.32±0.2, 1.69±0.12, 4.33±0.27 in KFb vs.1.43±0.13, 2.56±0.26, 2.89±0.37, 1.40±0.12 in NFb, P<0.05].ConclusionsThere are cell dysfunction and abnormal cellular dynamics in keloid fibroblasts. The formation of keloid likely involves aberrant interactions of some genes that affected its development at different extents.Key words: Keloid; Fibroblast; Cell cycle; Genes; Pathogenesis" @default.
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- W3032162007 date "2015-12-15" @default.
- W3032162007 modified "2023-09-27" @default.
- W3032162007 title "Proliferation, collagen production and related gene expression in keloids and normal skin fibroblasts" @default.
- W3032162007 doi "https://doi.org/10.3760/cma.j.issn.1671-0290.2015.06.013" @default.
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