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- W3032182988 abstract "ObjectiveTo investigate the effect of cyclosporine A (CsA) on autophagy-lysosomal pathway in tubular epithelial cells.MethodsHuman renal tubular epithelial cell line (HK-2 cell) was treated with different concentrations (3, 5 and 10 μmol/L) of CsA for 24 h. Then the viability and apoptosis of cells were measured by MTT assay or AnnexinV-PI staining followed by flow cytometry analysis, respectively. Autophagy-related protein LC3-Ⅱ and p62 were detected by immunofluorescence assay. Autophagic flux was analyzed in HK-2 cells transfected with a tandem mRFP-GFP fluorescent-tagged LC3 (tfLC3) plasmid by laser confocal microscope. The lysosomal degradation was evaluated by DQ-ovalbumin staining followed by flow cytometry analysis.ResultsThe viability of HK-2 cells was significantly decreased with CsA stimulation when compared with control group (P<0.01), but the number of apoptotic cells was markedly increased by CsA treatment (P<0.05). Compared with the control group, different doses of CsA dramatically increased the expressions of LC3-Ⅱ(P<0.01) and p62 (P<0.05) in HK-2 cells. Moreover, HK-2 cells treated with CsA displayed a significant increase in autophagosomes but a marked decrease in autolysosomes. In HK-2 cells, exposured to CsA caused a decrease in lysosomal degradation by DQ-ovalbumin staining when compared with control group (P<0.01).ConclusionBlockade of autophagy via disrupting lysosome degradation may represent a novel mechanism of CsA-induced tubular epithelial cells injury.Key words: Cyclosporine A; Epithelial cells; Kidney tubules; Autophagy; Lysosomes" @default.
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- W3032182988 date "2017-04-15" @default.
- W3032182988 modified "2023-09-23" @default.
- W3032182988 title "Effect of cyclosporine A on autophagy-lysosomal pathway in tubular epithelial cells" @default.
- W3032182988 doi "https://doi.org/10.3760/cma.j.issn.1001-7097.2017.04.007" @default.
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