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- W3032191036 abstract "ObjectiveTo explore the role of microRNA (miR)-24-3p in regulating the proliferation and migration of TPC1 cells.MethodsReverse transcriptase-polymerase chain reaction (RT-PCR) was applied to detect the expression of miR-24-3p in thyroid papillary carcinoma tissue and para-carcinoma tissue. The miR-24-3p mimic or negative control was transfected with Lipofectamine iMAX. The proliferation of TPC1 was measured using 5-ethynyl-2’-deoxyuridine (EdU) assays and cell counting kit-8 (CCK-8). The migration of TPC1 in vitro was determined using the wound healing and Transwell invasion assays. The expression of target mRNA and protein was detected. The luciferase report system was built to measure fluorescence value of firefly luciferase and sea renal fluorescence of each group.ResultsMiR-24-3p expression was down-regulated in thyroid papillary carcinoma tissue (0.28±0.02) as compared with para-carcinoma tissue (0.94±0.05, P=0.000). The miR-24-3p mimic inhibited the proliferation, migration and invasion of TPC1s [EdU: 19.3±2.5; CCK-8: 0.91±0.08; wound healing area: (23.6±3.5)%; number of migrating cells: 13.5±2.0] as compared with negative control group [EdU: 38.0±2.6, P=0.006; CCK-8: 1.81±0.08, P=0.001; wound healing area: (42.6±2.5)%, P=0.020; number of migrating cells: 31.3±2.5, P=0.040]. Western blotting results showed that platelet-derived growth factor receptor B (PDGFRB) protein was significantly down-regulated by transfection of miR-24-3p mimic (0.56±0.05) as compared with negative control group (1.19±0.12, P=0.020). RT-PCR results showed that PDGFRB mRNA levels were not affected significantly by transfection of miR-24-3p mimic (P=0.070). The luciferase report system proved that PDGFRB was a direct target of miR-24-3p.ConclusionMiR-24-3p can inhibit the proliferation and migration of TPC1 cells via directly targeting PDGFRB.Key words: Papillary thyroid carcinoma; MicroRNA; Platelet derived growth factor receptor B; MicroRNA-24-3p" @default.
- W3032191036 created "2020-06-05" @default.
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- W3032191036 date "2018-03-08" @default.
- W3032191036 modified "2023-09-27" @default.
- W3032191036 title "MicroRNA-24-3p inhibits the proliferation and migration of thyroid papillary cancer cells via platelet-derived growth factor receptor B" @default.
- W3032191036 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2018.03.012" @default.
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