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- W3032242410 abstract "Objective To study the role of MDC/CCL22-CCR4 axis in milky spots in peritoneal carcinomatosis of gastric cancer.Methods We examined the expression of CCR4 in 5 gastric cancer cell lines by RT-PCR and Western-blot.Cell growth rate of BGC-823 cell lines before and after incubated with CCL22 was detemined by MTT assay.The effects of CCL22 on invasion of BGC-823 cells into Matrigel were measured using Transwell test.Immunohistochemistry was conducted to detect the expression of CCR4.Results CCR4 mRNA and protein expression were detected in all 5 human gastric cancer cell lines:CCL22had a direct promotive effect on BGC-823 cell prolifetation and invasion.There was CCR4 expression in Stomach neoplasms.The expression of CCR4 in primary tumor was correlated with tumor pathological differentiation(P 0.05).Conclusions The MDC/CCL22-CCR4 axis may be one of the mechanism of peritoneal metastasis in milky spot in gastric cancer.Key words: Stomach neoplasms; Neoplasm metastasis; Chemotactic factors,macrophage; Milky spot" @default.
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- W3032242410 date "2010-04-25" @default.
- W3032242410 modified "2023-09-22" @default.
- W3032242410 title "MDC/CCL22-CCR4 axis in milky spots in peritoneal carcinomatosis of gastric cancer" @default.
- W3032242410 doi "https://doi.org/10.3760/cma.j.issn.1007-631x.2010.04.018" @default.
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