Matches in SemOpenAlex for { <https://semopenalex.org/work/W3032248914> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W3032248914 endingPage "402" @default.
- W3032248914 startingPage "395" @default.
- W3032248914 abstract "Objective To explore the values of potential clinical application ofsingle tube/ten colorsflow cytometry for leukocyte differential count in peripheral blood.Methods Utilizing multiple monoclonal antibody combinations and the vavious logical gating strategies,the tube/12 antibodies with no-wash method for the leukocyte differential count in peripheral blood were determined by using 10 colors flow cytometry.Leukocyte differentials of 142 peripheral blood samples were determined by both Beckman-Coulter LH750 hematology analyzer and 10 colors flow cytometry.The results were then compared to standard microscopic examination as a reference method.The clinical diagnostic efficiency ofsingle tube/10 colorsflow cytometry was calculated.The correlations between standard microscopic cytology,single tube/10 colorsflow cytometry and the hematology analyzer were determined.In addition,the clinical diagnosis efficiency for blast counts ofsingle tube/10 colorswere compared to the results determined by BD FACS Calibur flow cytometer.Results The leukocyte differentials were correlated well between the single tube/10 colorsflow cytometry and standard microscopic cytology(r>0.700,P<0.01) except for basophils.The correlations with neutrophilic granulocytes,lymphocytes,immature granulocytes and blasts were superior(r=0.972,0.951,0.801,0.912,respectively,P<0.01).When 1% was selected as the cut-off point for immature granulocytes determined by standard microscopic cytology,the sensitivity and the specificity ofsingle tube/10 colorsflow cytometry were 92%(57/62) and 79% (63/80),respectively.When 0.5% was selected as the cut-off point for blasts detected by standard microscopic cytology,the sensitivity and the specificity were 99% (67/68) and 92% (68/74).Using the immunophenotyping results from BD FACS Calibur as a standard,the sensitivity for detecting blasts bysingle tube/10 colOrsflow cytometry was 100% (40/40),the specificity was 91% (10/11),the positive predictive value was 98% (40/41),the negative predictive value was 100% (10/10) and the accuracy was 98% (50/51).Conclusions Thesingle tube/10 colorsflow cytometry has a excellent correlation with the standard microscopic cytology when applied on leukocyte differential count in peripheral blood.It may potentially use as a subsequent method for verification of abnormal results of complete blood cell count in the future.Key words: Leukocyte differential count; Flow cytometry; Blood cell count" @default.
- W3032248914 created "2020-06-05" @default.
- W3032248914 creator A5042782383 @default.
- W3032248914 creator A5044480186 @default.
- W3032248914 creator A5055526694 @default.
- W3032248914 creator A5075965720 @default.
- W3032248914 creator A5091332034 @default.
- W3032248914 date "2011-05-11" @default.
- W3032248914 modified "2023-09-24" @default.
- W3032248914 title "Leukocyte differential count in peripheral blood with single tube/ten colorsflow cytometry" @default.
- W3032248914 doi "https://doi.org/10.3760/cma.j.issn.1009-9158.2011.05.003" @default.
- W3032248914 hasPublicationYear "2011" @default.
- W3032248914 type Work @default.
- W3032248914 sameAs 3032248914 @default.
- W3032248914 citedByCount "0" @default.
- W3032248914 crossrefType "journal-article" @default.
- W3032248914 hasAuthorship W3032248914A5042782383 @default.
- W3032248914 hasAuthorship W3032248914A5044480186 @default.
- W3032248914 hasAuthorship W3032248914A5055526694 @default.
- W3032248914 hasAuthorship W3032248914A5075965720 @default.
- W3032248914 hasAuthorship W3032248914A5091332034 @default.
- W3032248914 hasConcept C142724271 @default.
- W3032248914 hasConcept C153911025 @default.
- W3032248914 hasConcept C1621761 @default.
- W3032248914 hasConcept C18823058 @default.
- W3032248914 hasConcept C203014093 @default.
- W3032248914 hasConcept C2780339063 @default.
- W3032248914 hasConcept C2993758460 @default.
- W3032248914 hasConcept C3017910909 @default.
- W3032248914 hasConcept C553184892 @default.
- W3032248914 hasConcept C71924100 @default.
- W3032248914 hasConcept C74864618 @default.
- W3032248914 hasConcept C86803240 @default.
- W3032248914 hasConceptScore W3032248914C142724271 @default.
- W3032248914 hasConceptScore W3032248914C153911025 @default.
- W3032248914 hasConceptScore W3032248914C1621761 @default.
- W3032248914 hasConceptScore W3032248914C18823058 @default.
- W3032248914 hasConceptScore W3032248914C203014093 @default.
- W3032248914 hasConceptScore W3032248914C2780339063 @default.
- W3032248914 hasConceptScore W3032248914C2993758460 @default.
- W3032248914 hasConceptScore W3032248914C3017910909 @default.
- W3032248914 hasConceptScore W3032248914C553184892 @default.
- W3032248914 hasConceptScore W3032248914C71924100 @default.
- W3032248914 hasConceptScore W3032248914C74864618 @default.
- W3032248914 hasConceptScore W3032248914C86803240 @default.
- W3032248914 hasIssue "05" @default.
- W3032248914 hasLocation W30322489141 @default.
- W3032248914 hasOpenAccess W3032248914 @default.
- W3032248914 hasPrimaryLocation W30322489141 @default.
- W3032248914 hasRelatedWork W135867231 @default.
- W3032248914 hasRelatedWork W1558223880 @default.
- W3032248914 hasRelatedWork W1965078249 @default.
- W3032248914 hasRelatedWork W1978910095 @default.
- W3032248914 hasRelatedWork W1979990228 @default.
- W3032248914 hasRelatedWork W2007114349 @default.
- W3032248914 hasRelatedWork W2028335258 @default.
- W3032248914 hasRelatedWork W203677436 @default.
- W3032248914 hasRelatedWork W2040663669 @default.
- W3032248914 hasRelatedWork W2055189506 @default.
- W3032248914 hasRelatedWork W2085896576 @default.
- W3032248914 hasRelatedWork W2156460410 @default.
- W3032248914 hasRelatedWork W2352176107 @default.
- W3032248914 hasRelatedWork W2382605575 @default.
- W3032248914 hasRelatedWork W2422776344 @default.
- W3032248914 hasRelatedWork W2538488673 @default.
- W3032248914 hasRelatedWork W2592213233 @default.
- W3032248914 hasRelatedWork W581670415 @default.
- W3032248914 hasRelatedWork W655587666 @default.
- W3032248914 hasRelatedWork W2741159246 @default.
- W3032248914 hasVolume "34" @default.
- W3032248914 isParatext "false" @default.
- W3032248914 isRetracted "false" @default.
- W3032248914 magId "3032248914" @default.
- W3032248914 workType "article" @default.