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- W3032270673 abstract "Objective To construct conditionally replicative adenovirus (CRAds) expressing short interference RNA targeting Ki67 gene (Ki67-sittNA) regulated by G250 promoter and p53-dysfunc-tion of tumor cells. Methods The siRNA expression cassettes of Ki67 gene containing H1 promoter were produced by PCR from pSilencer3, 1-Ki67 and inserted into pZD55, an E1B 55kD-deficient adenovirus vector to construct pZD55-Ki67-siRNA successfully. The E1A promoter of pZD55-Ki67-siRNA was re-placed by G250 promoter to construct pZD-G250-Ki67 successfully. The plasmid pZD-G250-Ki67 was transfected into 293 ceils together with plasmid pBHGE3 to obtain the recombinant CRAds, ZD-G250-Ki67. The viral plaques appeared 9-12 days after infection. The recombinant adenoviruses were verified by PCR. Viruses were plaque purified, propagated on HEK293 cells and functional PFU titers were determined by plaque assay on 293 cells. Results CRAds expressing Ki67-siRNA gene regulated by G250 promoter and p53-dysfunction of tumor cells had been constructed successfully. Viral PFU titers were 2 × 101 1 PFU/ml. Conclusion The CRAds constructed may be used for further investigation on Gene-Viro therapy of re-nal carcinoma.Key words: Renal carcinoma; Promoter; Adenovirus; RNA interference" @default.
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- W3032270673 date "2009-10-08" @default.
- W3032270673 modified "2023-09-23" @default.
- W3032270673 title "Construction and identification of conditionally replicative adenovirus expressing short interference RNA targeting Ki67 gene dually regulated by G250 promoter and p53-dysfunction of tumor cells" @default.
- W3032270673 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2009.10.010" @default.
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