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- W3032392165 abstract "Objective To compare serological methods with real time RT-PCR in detection of severe fever with thrombocytopenia syndrome Bunyavirus.Method Serum of severe fever with thrombocytopenia syndrome was collected in Penglai and Laizhou in Yantai.Anti-severe fever with thrombocytopenia syndrome Bunyavirus (SFTSV) IgM was detected using gold colloid and ELISA,nucleic acid of severe fever with thrombocytopenia syndrome Bunyavirus was detected by real-time PCR.Statistics of the results were done using SPSS 18.0.Result The coincidence of ELISA and gold colloid was 96.97% and the kcoefficient was 0.933 ;the course of disease influences the results notablely both gold colloid and real-time PCR(P < 0.05) and real-time PCR was not influenced by patients age or sex(P > 0.05).Conclusion When the course of disease less than 12 days,real-time PCR was recommended and when the course of disease more than 16 days,serological methods can use to detect the anti-SFTSV IgM.Key words: Serological test; Gold Colloid; Enzyme-linked immunosorbent assay; Polyomerase chain reaction; Bunyavirus" @default.
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- W3032392165 date "2013-12-30" @default.
- W3032392165 modified "2023-09-23" @default.
- W3032392165 title "Comparison of serological methods and real time RT-PCR in detection of severe fever with thrombocytopenia syndrome Bunyavirus" @default.
- W3032392165 doi "https://doi.org/10.3760/cma.j.issn.1003-9279.2013.06.031" @default.
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