Matches in SemOpenAlex for { <https://semopenalex.org/work/W3032426078> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W3032426078 endingPage "78" @default.
- W3032426078 startingPage "75" @default.
- W3032426078 abstract "目的:建立小鼠多药耐药白血病细胞系P388/VCR并研究其生物学特性.方法:小鼠淋巴细胞白血病细胞系P388,通过长期、单一、逐步提高浓度的方法建立多药耐药的白血病细胞系P388/VCR.采用悬浮细胞培养和半固体培养观察该细胞系的生长规律;流式细胞术分析细胞周期分布及柔红霉素在细胞内的积聚量;RT-PCR方法检测mdrla基因表达;四氮甲基唑蓝(MTT)法研究P388/VCR细胞系的耐药谱.结果:经过6个月单一、小剂量及间歇给药的方法诱导P388细胞成为多药耐药细胞系P388/VCR.与亲本细胞系P388比较,细胞生长曲线及倍增时间无明显差异,软琼脂培养集落形成率及大集落形成率差异不显著,细胞周期分析P388/VCR细胞S期比例降低.P388/VCR细胞除对原诱导药物具有耐药性外,对其他多种抗肿瘤药物如柔红霉素、高三尖杉酯碱、米托蒽醌、足叶乙苷等也具有交叉耐药性.流式细胞仪检测细胞内柔红霉素含量P388/VCR细胞平均荧光值为3.72,明显低于P388细胞的1731.RT-PCR检测mdrla基因的表达结果为:P388细胞阴性,P388/VCR细胞阳性.结论:多药耐药细胞系P388/VCR具有mdrla基因表达阳性及对多种抗肿瘤药物耐药的特征。" @default.
- W3032426078 created "2020-06-05" @default.
- W3032426078 creator A5005839836 @default.
- W3032426078 creator A5016958640 @default.
- W3032426078 creator A5032903359 @default.
- W3032426078 creator A5074039086 @default.
- W3032426078 creator A5080730703 @default.
- W3032426078 creator A5083357246 @default.
- W3032426078 creator A5086281775 @default.
- W3032426078 date "2004-04-25" @default.
- W3032426078 modified "2023-10-15" @default.
- W3032426078 title "ESTABLISHMENT AND CHARACTERISTICS OF MURINE MULTI- DRUG RESISTANT LEUKEMIA CELL LINE" @default.
- W3032426078 doi "https://doi.org/10.3760/cma.j.issn.1009-9921.2004.02.004" @default.
- W3032426078 hasPublicationYear "2004" @default.
- W3032426078 type Work @default.
- W3032426078 sameAs 3032426078 @default.
- W3032426078 citedByCount "0" @default.
- W3032426078 crossrefType "journal-article" @default.
- W3032426078 hasAuthorship W3032426078A5005839836 @default.
- W3032426078 hasAuthorship W3032426078A5016958640 @default.
- W3032426078 hasAuthorship W3032426078A5032903359 @default.
- W3032426078 hasAuthorship W3032426078A5074039086 @default.
- W3032426078 hasAuthorship W3032426078A5080730703 @default.
- W3032426078 hasAuthorship W3032426078A5083357246 @default.
- W3032426078 hasAuthorship W3032426078A5086281775 @default.
- W3032426078 hasConcept C203014093 @default.
- W3032426078 hasConcept C2778461978 @default.
- W3032426078 hasConcept C2780035454 @default.
- W3032426078 hasConcept C502942594 @default.
- W3032426078 hasConcept C54355233 @default.
- W3032426078 hasConcept C81885089 @default.
- W3032426078 hasConcept C86803240 @default.
- W3032426078 hasConcept C98274493 @default.
- W3032426078 hasConceptScore W3032426078C203014093 @default.
- W3032426078 hasConceptScore W3032426078C2778461978 @default.
- W3032426078 hasConceptScore W3032426078C2780035454 @default.
- W3032426078 hasConceptScore W3032426078C502942594 @default.
- W3032426078 hasConceptScore W3032426078C54355233 @default.
- W3032426078 hasConceptScore W3032426078C81885089 @default.
- W3032426078 hasConceptScore W3032426078C86803240 @default.
- W3032426078 hasConceptScore W3032426078C98274493 @default.
- W3032426078 hasIssue "02" @default.
- W3032426078 hasLocation W30324260781 @default.
- W3032426078 hasOpenAccess W3032426078 @default.
- W3032426078 hasPrimaryLocation W30324260781 @default.
- W3032426078 hasRelatedWork W1988924915 @default.
- W3032426078 hasRelatedWork W2087874471 @default.
- W3032426078 hasRelatedWork W2348587804 @default.
- W3032426078 hasRelatedWork W2350094712 @default.
- W3032426078 hasRelatedWork W2362023821 @default.
- W3032426078 hasRelatedWork W2369316205 @default.
- W3032426078 hasRelatedWork W2372403516 @default.
- W3032426078 hasRelatedWork W2377783865 @default.
- W3032426078 hasRelatedWork W2380763427 @default.
- W3032426078 hasRelatedWork W2380887829 @default.
- W3032426078 hasRelatedWork W2384483070 @default.
- W3032426078 hasRelatedWork W2385913749 @default.
- W3032426078 hasRelatedWork W238766373 @default.
- W3032426078 hasRelatedWork W2390734888 @default.
- W3032426078 hasRelatedWork W2397135745 @default.
- W3032426078 hasRelatedWork W2409205014 @default.
- W3032426078 hasRelatedWork W2907998891 @default.
- W3032426078 hasRelatedWork W3032738973 @default.
- W3032426078 hasRelatedWork W312153725 @default.
- W3032426078 hasRelatedWork W2594467738 @default.
- W3032426078 hasVolume "13" @default.
- W3032426078 isParatext "false" @default.
- W3032426078 isRetracted "false" @default.
- W3032426078 magId "3032426078" @default.
- W3032426078 workType "article" @default.