Matches in SemOpenAlex for { <https://semopenalex.org/work/W3032465069> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W3032465069 endingPage "774" @default.
- W3032465069 startingPage "770" @default.
- W3032465069 abstract "ObjectiveTo assess the ability of simultaneous amplification and testing (SAT) in bacteria detection, bronchoalveolar lavage fluid from children was collected and detected by SAT, combined with in vitro culture experiment, providing theoretical support for the application of SAT in the diagnosis and treatment of mycoplasma pneumonia (MP).MethodsA total of 572 bronchoalveolar lavage fluid from children with community acquired pneumonia during October 2015 and December 2017 in Tianjin Children′s Hospital were collected and detected by Mycoplasma pneumonia(MP)nucleic acid quantitative assay and SAT technology. Among them, 161 bronchoalveolar lavage fluid were also detected using in vitro culture and the positive ones for MP were analyzed by nucleic acid quantification and SAT after exposing to high concentration of antibiotic.ResultsThe positive rates of MP by nucleic acid quantitative assay and SAT technology in 572 samples were 74.7% (427/572) and 71.9% (411/572), respectively. These two detection methods have high consistency (χ2=1.142, P=0.285). According to the test results of SAT, the positive rates of male and female were 72.7% (224/308) and 70.8% (187/264), respectively. There was no significant difference of positive rate between different sex (χ2=0.252, P=0.616). The positive rate of MP in 4-14 years old children (78.1%, 317/416) was higher than that in infants (≤3 years) (56.6%, 94/166) (χ2=26.811, P=0.000). After adding azithromycin (5 MIC) to MP positive medium for 5 days, the result of nucleic acid quantification was positive but SAT was negative.ConclusionsSAT technology is a rapid, sensitive and specific method for detection of MP. In addition, SAT technology could identify the dead and live bacteria and could evaluate the effect of clinical treatment effectively.(Chin J Lab Med, 2018, 41: 770-774)Key words: Mycoplasma pneumoniae; Bronchoalveolar lavage fluid; Nucleic acid amplification techniques; Microbial sensitivity tests" @default.
- W3032465069 created "2020-06-05" @default.
- W3032465069 creator A5009322394 @default.
- W3032465069 creator A5028986244 @default.
- W3032465069 creator A5046597133 @default.
- W3032465069 creator A5055838753 @default.
- W3032465069 creator A5080203487 @default.
- W3032465069 date "2018-10-11" @default.
- W3032465069 modified "2023-09-26" @default.
- W3032465069 title "Detection and clinical application of the isothermal amplification of Mycoplasma pneumonia RNA in bronchoalveolar lavage fluid from children" @default.
- W3032465069 doi "https://doi.org/10.3760/cma.j.issn.1009-9158.2018.10.013" @default.
- W3032465069 hasPublicationYear "2018" @default.
- W3032465069 type Work @default.
- W3032465069 sameAs 3032465069 @default.
- W3032465069 citedByCount "0" @default.
- W3032465069 crossrefType "journal-article" @default.
- W3032465069 hasAuthorship W3032465069A5009322394 @default.
- W3032465069 hasAuthorship W3032465069A5028986244 @default.
- W3032465069 hasAuthorship W3032465069A5046597133 @default.
- W3032465069 hasAuthorship W3032465069A5055838753 @default.
- W3032465069 hasAuthorship W3032465069A5080203487 @default.
- W3032465069 hasConcept C126322002 @default.
- W3032465069 hasConcept C203014093 @default.
- W3032465069 hasConcept C24107716 @default.
- W3032465069 hasConcept C2777714996 @default.
- W3032465069 hasConcept C2777914695 @default.
- W3032465069 hasConcept C2777961210 @default.
- W3032465069 hasConcept C2778838687 @default.
- W3032465069 hasConcept C2779215961 @default.
- W3032465069 hasConcept C2779706735 @default.
- W3032465069 hasConcept C55493867 @default.
- W3032465069 hasConcept C71924100 @default.
- W3032465069 hasConcept C86803240 @default.
- W3032465069 hasConcept C89423630 @default.
- W3032465069 hasConcept C90924648 @default.
- W3032465069 hasConceptScore W3032465069C126322002 @default.
- W3032465069 hasConceptScore W3032465069C203014093 @default.
- W3032465069 hasConceptScore W3032465069C24107716 @default.
- W3032465069 hasConceptScore W3032465069C2777714996 @default.
- W3032465069 hasConceptScore W3032465069C2777914695 @default.
- W3032465069 hasConceptScore W3032465069C2777961210 @default.
- W3032465069 hasConceptScore W3032465069C2778838687 @default.
- W3032465069 hasConceptScore W3032465069C2779215961 @default.
- W3032465069 hasConceptScore W3032465069C2779706735 @default.
- W3032465069 hasConceptScore W3032465069C55493867 @default.
- W3032465069 hasConceptScore W3032465069C71924100 @default.
- W3032465069 hasConceptScore W3032465069C86803240 @default.
- W3032465069 hasConceptScore W3032465069C89423630 @default.
- W3032465069 hasConceptScore W3032465069C90924648 @default.
- W3032465069 hasIssue "10" @default.
- W3032465069 hasLocation W30324650691 @default.
- W3032465069 hasOpenAccess W3032465069 @default.
- W3032465069 hasPrimaryLocation W30324650691 @default.
- W3032465069 hasRelatedWork W2014582252 @default.
- W3032465069 hasRelatedWork W2037139653 @default.
- W3032465069 hasRelatedWork W2043347741 @default.
- W3032465069 hasRelatedWork W2097856873 @default.
- W3032465069 hasRelatedWork W2146245572 @default.
- W3032465069 hasRelatedWork W2166999132 @default.
- W3032465069 hasRelatedWork W2362471468 @default.
- W3032465069 hasRelatedWork W2363678005 @default.
- W3032465069 hasRelatedWork W2375464420 @default.
- W3032465069 hasRelatedWork W2385134374 @default.
- W3032465069 hasRelatedWork W2406388004 @default.
- W3032465069 hasRelatedWork W2417090879 @default.
- W3032465069 hasRelatedWork W2802906476 @default.
- W3032465069 hasRelatedWork W2963736356 @default.
- W3032465069 hasRelatedWork W3031557509 @default.
- W3032465069 hasRelatedWork W3031776781 @default.
- W3032465069 hasRelatedWork W3049609233 @default.
- W3032465069 hasRelatedWork W3110191365 @default.
- W3032465069 hasRelatedWork W3194962655 @default.
- W3032465069 hasRelatedWork W1902402451 @default.
- W3032465069 hasVolume "41" @default.
- W3032465069 isParatext "false" @default.
- W3032465069 isRetracted "false" @default.
- W3032465069 magId "3032465069" @default.
- W3032465069 workType "article" @default.