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- W3032486511 abstract "Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detection system that is useful for detecting multiple species of mycoplasma in infected cell lines. The system contains three dye-labeled detection aptamers that can specifically bind to mycoplasma-infected cells and a dye-labeled control aptamer that minimally binds to cells. With this system, mycoplasma-contaminated cells can be detected within 30 min by using a flow cytometer, fluorescence microscope, or microplate reader. Further, this system may be used to detect mycoplasma-contaminated culture medium. This study presents an novel mycoplasma detection model that is simple, rapid, inexpensive, and sensitive." @default.
- W3032486511 created "2020-06-05" @default.
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- W3032486511 date "2020-05-27" @default.
- W3032486511 modified "2023-10-17" @default.
- W3032486511 title "Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture" @default.
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- W3032486511 doi "https://doi.org/10.3390/ijms21113784" @default.
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