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- W3032566773 abstract "ObjectiveTo observe the expression and distribution of NLRP3 in rats brain and to explore the significant correlations between NLRP3 and cerebral ischemia/reperfusion(IR) injuries.MethodsForty Male SD rats were randomly divided into the Sham group, ischemia/reperfusion 12h, 24h, 48h group. The ischemia model was induced by occlusion of the middle cerebral artery (MCAO) with a microfilament in rats. The expression and distribution of NLRP3 in rats brain was explored by immunofluorescence staining. After reperfusion injury, we examined the levels of protein and mRNA by Western blot and real-time PCR, respectively. Another 40 Male SD rats were randomly divided into the sham-control group, IR-control group, sham-glyburine group, IR-glyburine group. Glyburine used as inhibitor of NLRP3 was given to the rats in glyburine group before MCAO model. In order to analyze infarct and assess function of blood-brain barrier (BBB), magnetic resonance imaging(MRI) and Evans blue stain were performed.ResultsNLRP3 was mainly expressed in microglia and endothelial cells by immunofluorescence staining. By real-time PCR and Western blot analyses, NLRP3 expression was markedly enhanced in the ischemic cerebral hemisphere with a peak expression of NLRP3 at 24 hours after reperfusion(P< 0.05). In MRI the area of treatment/reperfusion group was significantly smaller than? control/reperfusion group[(21.7±4.2)% vs. (36.0 ±4.7)%, (P< 0.05). Our results also indicated that the content of Evans blue of treatment/reperfusion? group was significantly lower than control/reperfusion group[(18.7 ± 3.8)μg/g vs. (32.1 ± 5.1)μg/g]by Evans blue stain(P< 0.05).ConclusionsNLRP3 was mainly expressed in microglia and endothelial cells. NLRP3 contributes to the development of cerebral ischemia/reperfusion injury and ischemia-induced BBB disruption.Key words: Brain ischemia; Reperfusion injury; Blood-brain barrier; Rats; NLRP3 inflammasome" @default.
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- W3032566773 date "2015-01-28" @default.
- W3032566773 modified "2023-09-29" @default.
- W3032566773 title "NLRP3 inflammasome deficiency reduces rats brain damage after cerebral ischemia reperfusion" @default.
- W3032566773 doi "https://doi.org/10.3760/cma.j.issn.1001-2346.2015.01.019" @default.
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