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- W3032644438 abstract "ObjectiveTo investigate the effect of xanthohumol on the biological activity of prolactinoma MMQ cells.MethodsMMQ cells were divided into drug group and control group. The cells in drug group were treated with various concentrations of xanthohumol (low dose: 0.1μmol/L, middle dose: 1μmol/L, high dose: 10 μmol/L), and those in control group was treated with equal volume of dimethyl sulfoxide (DMSO). The proliferation of MMQ cells was determined by MTS experiment, and the apoptosis levels by Annexin V/PI and reactive oxygen species (ROS) levels by DCFH-DA experiment. The expressions of genes and proteins related to invasion were measured by RT-PCR and western blot.ResultsXanthohumol effectively inhibited the proliferation of MMQ in a dose-dependent and time-dependent manner. The cell viability was reduced 4%, 15% and 19% after 24 hours of xanthohumol treatment, and 8%, 18% and 36% after 48 hours, and 17%, 38% and 52% after 72 hours. After 24 hours of xanthohumal treatment, Annexin V positive cells accounted for 4.2%, 12.1% and 16.7% among total cells, and propidium iodide (PI) positive cells were 3.8%, 6.7% and 9.2%. The mRNA levels of E-Cadherin in drug group were 1.34±0.43, 2.93±0.78 and 5.33±1.21 folds of those in control group, and protein levels of E-Cadherin were 1.80±0.16, 3.20±0.45 and 3.6±0.53 times higher than those in control (P<0.01). The mRNA levels of VEGF (vascular endothelial growth factor) in drug group was 0.79±0.37, 0.33±0.17 and 0.18±0.12 folds of those in control group, and the protein levels were 0.52±0.11, 0.43±0.13 and 0.22±0.07 folds of those in control (P<0.01). Xanthohumol significantly increased ROS in MMQ cells, and the mean fluorescence intensity in high-dose group was approximately 3.40±0.56 folds of that in control (P<0.01).ConclusionXanthohumol could induce apoptosis and inhibit the expression of genes related to invasion of MMQ cells, which involves the mitochondrial dysfunction.Key words: Prolactinoma; Apoptosis; Reactive oxygen species; Xanthohumol" @default.
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- W3032644438 date "2018-05-28" @default.
- W3032644438 modified "2023-09-24" @default.
- W3032644438 title "The effect of xanthohumol on the biological activity of prolactinoma MMQ cells" @default.
- W3032644438 doi "https://doi.org/10.3760/cma.j.issn.1001-2346.2018.05.020" @default.
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