FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3032698889> ?p ?o ?g. }

• W3032698889 endingPage "897" @default.
• W3032698889 startingPage "892" @default.
• W3032698889 abstract "ObjectiveTo explore the pathogenesis of di-n-butyl phthalate (DBP)-induced hypospadias, detect the differential expression and activity of steroidogenic acute regulatory (StAR) protein in fetal testes and measure the testosterone concentration in sera.MethodsForty pregnant Sprague Dawley rats were randomly divided into two groups (n=20 each). Dams were treated by gavage at 9 a. m daily from gestation day (GD) 13 to GD19 with corn oil vehicle (3 ml/kg) or DBP in corn oil at 800 mg/kg (total DBP & corn oil at 3 ml/kg). On GD19 at 12 a. m, fetuses were immediately removed from uterus and sexed by internal examination of reproductive organs. Hypospadiac and normal rats were randomly divided into hypospadiac and control groups. Outer appearance of genital tubercles(GT) was examined. And anogenital distance (AGD) was measured. The incidence of hypospadias was calculated. And GT was stained with hematoxylin & eosin (H&E). The concentration of testosterone was measured with enzyme-linked immunosorbent assay (ELISA) kits. Testes were removed from male fetuses. Real-time quantitative polymerase chain reaction (PCR), Western blot, ELISA and immunofluorescence were used for measuring the expression of StAR mRNA or StAR protein in testes. Pearson's correlation was used for analyzing the relationship between relative expression of StAR protein and testosterone concentration. Western blot was utilized for quantifying the expression of T-ERK1/2 and P-ERK1/2 in testes. To determine the activity of StAR protein, ERK1/2 phosphorylation was analyzed by the ratio of P-ERK1/2/T-ERK1/2.ResultsHE incidence of hypospadias at 43.3%(46/106). The testosterone concentration was also lower in hypospadiac group than that in control group [(1.45±0.62) ng/ml vs. (4.48±0.93) ng/ml, P<0.05]. Significant down-regulation of StAR was also found in both mRNA and protein in hypospadiac group (StAR mRNA: 0.23±0.08; StAR: 0.33±0.07) when compared with control group (StAR mRNA: 1.00±0.00; StAR: 1.44±0.19, P<0.05). StAR protein was located predominantly in interstitial cell of tests and staining intensity was obviously weaker in hypospadiac group than that in control group. Pearson’s correlation coefficient was also lower in hypospadiac group (r=0.642, P<0.05) than that in control group (r=0.851, P<0.05). The ratio of P-ERK1/2/ERK1/2 (P-ERK1/ERK1: 0.17±0.03; P-ERK2/ERK2: 0.19±0.07) significantly decreased in hypospadiac group as compared with control group (P-ERK1/ERK1: 0.31±0.08; P-ERK2/ERK2: 0.43±0.14, P<0.05).ConclusionsDBP not only decreases the expression of StAR at the levels of gene and protein translation, but also inhibits the activity of StAR protein by decreasing the level of ERK1/2 phosphorylation to affect the synthesis of testosterone, thereby inducing hypospadias.Key words: Hypospadias; Laboratory animal; Phthalate; Testosterone" @default.
• W3032698889 created "2020-06-05" @default.
• W3032698889 creator A5021616613 @default.
• W3032698889 creator A5061025784 @default.
• W3032698889 creator A5086330863 @default.
• W3032698889 date "2017-12-15" @default.
• W3032698889 modified "2023-09-28" @default.
• W3032698889 title "Differential expression and activity of steroidogenic acute regulatory protein in testes of hypospadiac male rats with in utero exposure to di-n-butyl phthalate" @default.
• W3032698889 doi "https://doi.org/10.3760/cma.j.issn.0253-3006.2017.12.004" @default.
• W3032698889 hasPublicationYear "2017" @default.
• W3032698889 type Work @default.
• W3032698889 sameAs 3032698889 @default.
• W3032698889 citedByCount "0" @default.
• W3032698889 crossrefType "journal-article" @default.
• W3032698889 hasAuthorship W3032698889A5021616613 @default.
• W3032698889 hasAuthorship W3032698889A5061025784 @default.
• W3032698889 hasAuthorship W3032698889A5086330863 @default.
• W3032698889 hasConcept C104317684 @default.
• W3032698889 hasConcept C105702510 @default.
• W3032698889 hasConcept C126322002 @default.
• W3032698889 hasConcept C134018914 @default.
• W3032698889 hasConcept C150194340 @default.
• W3032698889 hasConcept C16685009 @default.
• W3032698889 hasConcept C172680121 @default.
• W3032698889 hasConcept C178790620 @default.
• W3032698889 hasConcept C185592680 @default.
• W3032698889 hasConcept C201614185 @default.
• W3032698889 hasConcept C2776415932 @default.
• W3032698889 hasConcept C2777668474 @default.
• W3032698889 hasConcept C2777990738 @default.
• W3032698889 hasConcept C2778206080 @default.
• W3032698889 hasConcept C2779234561 @default.
• W3032698889 hasConcept C2779279991 @default.
• W3032698889 hasConcept C2779670467 @default.
• W3032698889 hasConcept C2781005124 @default.
• W3032698889 hasConcept C46973012 @default.
• W3032698889 hasConcept C54355233 @default.
• W3032698889 hasConcept C55493867 @default.
• W3032698889 hasConcept C71924100 @default.
• W3032698889 hasConcept C86803240 @default.
• W3032698889 hasConceptScore W3032698889C104317684 @default.
• W3032698889 hasConceptScore W3032698889C105702510 @default.
• W3032698889 hasConceptScore W3032698889C126322002 @default.
• W3032698889 hasConceptScore W3032698889C134018914 @default.
• W3032698889 hasConceptScore W3032698889C150194340 @default.
• W3032698889 hasConceptScore W3032698889C16685009 @default.
• W3032698889 hasConceptScore W3032698889C172680121 @default.
• W3032698889 hasConceptScore W3032698889C178790620 @default.
• W3032698889 hasConceptScore W3032698889C185592680 @default.
• W3032698889 hasConceptScore W3032698889C201614185 @default.
• W3032698889 hasConceptScore W3032698889C2776415932 @default.
• W3032698889 hasConceptScore W3032698889C2777668474 @default.
• W3032698889 hasConceptScore W3032698889C2777990738 @default.
• W3032698889 hasConceptScore W3032698889C2778206080 @default.
• W3032698889 hasConceptScore W3032698889C2779234561 @default.
• W3032698889 hasConceptScore W3032698889C2779279991 @default.
• W3032698889 hasConceptScore W3032698889C2779670467 @default.
• W3032698889 hasConceptScore W3032698889C2781005124 @default.
• W3032698889 hasConceptScore W3032698889C46973012 @default.
• W3032698889 hasConceptScore W3032698889C54355233 @default.
• W3032698889 hasConceptScore W3032698889C55493867 @default.
• W3032698889 hasConceptScore W3032698889C71924100 @default.
• W3032698889 hasConceptScore W3032698889C86803240 @default.
• W3032698889 hasIssue "12" @default.
• W3032698889 hasLocation W30326988891 @default.
• W3032698889 hasOpenAccess W3032698889 @default.
• W3032698889 hasPrimaryLocation W30326988891 @default.
• W3032698889 hasRelatedWork W1591843654 @default.
• W3032698889 hasRelatedWork W1916271309 @default.
• W3032698889 hasRelatedWork W1958707635 @default.
• W3032698889 hasRelatedWork W2002680564 @default.
• W3032698889 hasRelatedWork W2013494417 @default.
• W3032698889 hasRelatedWork W2148029218 @default.
• W3032698889 hasRelatedWork W2353432038 @default.
• W3032698889 hasRelatedWork W2355178851 @default.
• W3032698889 hasRelatedWork W2360466244 @default.
• W3032698889 hasRelatedWork W2371559760 @default.
• W3032698889 hasRelatedWork W2373015108 @default.
• W3032698889 hasRelatedWork W2374062742 @default.
• W3032698889 hasRelatedWork W2378310448 @default.
• W3032698889 hasRelatedWork W2379427602 @default.
• W3032698889 hasRelatedWork W2379836766 @default.
• W3032698889 hasRelatedWork W2382435072 @default.
• W3032698889 hasRelatedWork W2382909526 @default.
• W3032698889 hasRelatedWork W2392972818 @default.
• W3032698889 hasRelatedWork W2517268195 @default.
• W3032698889 hasRelatedWork W3030411271 @default.
• W3032698889 hasVolume "38" @default.
• W3032698889 isParatext "false" @default.
• W3032698889 isRetracted "false" @default.
• W3032698889 magId "3032698889" @default.
• W3032698889 workType "article" @default.