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- W3032709543 abstract "Objectives Observe and analyze the effect of A20 transfection on damage and apoptosis of renal tubular in rats induced by renal ischemia-reperfusion.Methods Constructing anthropogenic A20 gene expression vector pcDNA3.1-A20 and empty plasmid vector pcDNA3.1,wrapped with liposome.Twenty-four male SD rats were randomly divided into three groups (n =8):saline group,A20 group,empty plasmid group.Forty-eight hours before operation every group was injected with Saline 250ul,liposome pcDNA3.1-A20 + saline to 250ul,empty plastid-PcDNA3.1 + saline to 250ul,respectively.then renal ischemia reperfusion injury model was done by clamping left renal artery and vein for 45 min,and cut the right renal before reperfusion of left renal vascular.Twenty-four hours after operation harvested the rats,then the renal function was detected by automatic biochemical analyzer,renal pathological examination was analyzed by HE staining,renal tubular cell apoptosis was detected by TUNEL assay.Results Serum Scr and BUN level of A20 group was lower than the other two groups,renal pathological damage was alleviated significantly,renal tubular epithelial cell apoptosis was decreased.Conclusions A20 transfection can significantly reduce the renal IRI,which may be related to the inhibition of apoptosis of renal tubular epithelial cells.Key words: Reperfusion Injury ; Apoptosis ; Kidney Tubules ; Rats ;" @default.
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- W3032709543 date "2013-09-15" @default.
- W3032709543 modified "2023-09-25" @default.
- W3032709543 title "Effect of A20 transfection on apoptosis of renal tubuler epithelial cell in rats induced by renal ischemia reperfusion injury" @default.
- W3032709543 doi "https://doi.org/10.3760/cma.j.issn.1673-4416.2013.05.003" @default.
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