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• W3032737932 abstract "ObjectiveTo investigate both in mechanism of hyperoxia-induced acute lung injury(HALI) by vivo experiment, to observe the Bruton’s tyrosine kinase (Btk) and nuclear factor kappa B (NF-кB) signals expression level.MethodsTotal of 72 healthy male Kunming mice were randomly(random number)divided into four groups: air control group, hyperoxia exposure 3 days group (H3d group), hyperoxia exposure 3 days + inhibitor group(H3d + I group) and inhibitor groups . Then the pathological changes of lung tissues were observed under light microscope; The total protein content (TP) of bronchoalveolar lavage fluid (BALF)and wet/dry weight ratio(W/D) of lung were detected; The protein expression of Btk, p-Btk, pNF-кB p65 were mersured by Western blot; the mRNA level of IL-6 was determined by real-time polymerase chain reaction(qRT-PCR); the level of monocyte chemoattractant protein-1(MCP-1)in serum was detected by enzyme-linked immunosorbent assay(ELISA) .Statistcal significance was determined by 1-way ANOVA.ResultsThere were no significant difference in the data between the control group and the inhibitor group(P>0.05). The pathological injury in light microscope, content of total protein in BALF, W/D ratio of lung tissues in H3d group were significantly higher than H3d+ I group(Respectively P=0.002, P=0.000 ). Western blot analysis showed that expression of Btk, p-Btk, pNF-кB p65 in H3d group were significantly higher than those in H3d+ I group(Respectively P=0.002, P=0.013, P=0.000). RT-qPCR results showed that the expression of IL-6 mRNA in H3d group were significantly higher than control group(P=0.004), inhibitor group (P=0.000) and H3d+ I group(P=0.021). In addition, The serum MCP-1 levels in H3d group were higher markely than the control group (P=0.002), inhibitor group (P=0.000) and H3d+ I group (P=0.009). The correlation analysis showed that pNF-кB p65 were positively correlated wiht Btk and p-Btk (r=0.902 and 0.954, P<0.01).ConclusionsBtk may trigger the release of IL-6 and MCP-1 by mediating the signaling pathway of NF-кB in vivo study, which was most important in the occurrence of HALI.Therefore, inhibiting the Btk activity would alleviate the severity of lung injury effectively.Key words: Hyperoxia; Acute lung injury; Bruton’s tyrosine kinase; Nuclear factor-kappa B; Mice; Wet/dry weight ratio; Monocyte chemoattractant protein-1; Signaling pathway" @default.
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• W3032737932 date "2017-02-10" @default.
• W3032737932 modified "2023-09-25" @default.
• W3032737932 title "Effect of Bruton’s tyrosine kinase in hyperoxia-induced acute lung injury" @default.
• W3032737932 doi "https://doi.org/10.3760/cma.j.issn.1671-0282.2017.02.012" @default.
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