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- W3032743509 abstract "目的 了解E-selectin、Integrin β1、ICAM-1在人胃癌细胞中的表达水平,探讨胃癌细胞、正常胃上皮细胞与人脐静脉内皮细胞的黏附力学特性及其黏附分子的作用机制.方法 采用Northern印迹法和ELISA法检测胃癌细胞、正常胃上皮细胞和人脐静脉内皮细胞E-selectin、Integrin β1及ICAM-1表达水平;采用微管吸吮技术分别测量了胃癌细胞、正常胃上皮细胞与人脐静脉内皮细胞间的黏附特性,利用抗体阻断实验验证胃腺癌细胞与人脐静脉内皮细胞黏附行为中E-selectin、Integrin β1及ICAM-1的作用.结果 胃癌细胞、正常胃上皮细胞和人脐静脉内皮细胞均有E-selectin、Integrin β1及ICAM-1表达,但胃癌细胞3种黏附分子表达水平均高于正常胃上皮细胞和人脐静脉内皮细胞,差异有统计学意义(P＜0.05).胃癌细胞与人脐静脉内皮细胞的黏附力值为(31±4)×10-10 N,较之正常胃上皮细胞黏附力明显升高[(20±3)×10-10 N],两者差异有统计学意义(P＜0.001).抗体阻断前后细胞之间的黏附力比较差异有统计学意义(P＜0.05).结论 E-selectin、Integrin β1及ICAM-1可能与胃癌细胞转移有关。" @default.
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- W3032743509 date "2007-02-20" @default.
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- W3032743509 title "Expressions of E-selectin, Integrin β1 and ICAM-1 in gastric cancer and adhesive properties of gastric cancer cells to umbilical vein endothelial cells" @default.
- W3032743509 doi "https://doi.org/10.3760/cma.j.issn.1673-9752.2007.01.023" @default.
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