FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3032795231> ?p ?o ?g. }

• W3032795231 endingPage "591" @default.
• W3032795231 startingPage "586" @default.
• W3032795231 abstract "ObjectiveTo investigate the role of Toll like receptor 4/nuclear factor-κB (TLR4/NF-κB) signal pathway on myocardial dysfunction after cardiac arrest-cardiopulmonary resuscitation (CA-CPR) in animal model.MethodsTwenty-six pigs were randomly divided into sham group (n = 6), CA-CPR 12 hours group (n = 10) and CA-CPR 24 hours group (n = 10). The model of CA-CPR was reproduced by endocardial electrical stimulation for 8 minutes followed by CPR, and the pigs in sham group were only given anesthesia and tracheal intubation. The changes in hemodynamics including mean arterial pressure (MAP) and cardiac output (CO), as well as morphology and ultrastructure of myocardial cells were observed before and after CPR. The levels of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined by enzyme linked immunosorbent assay (ELISA), and protein and mRNA expressions of TLR4/NF-κB in the myocardium were determined by Western Blot and real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR), respectively.ResultsHemodynamic disturbance and myocardial serious injury were observed in CA-CPR groups. Compared with sham group, the levels of serum TNF-α were markedly increased 0.5 hour after return of spontaneous circulation (ROSC) in CA-CPR 12 hours and 24 hours groups (pg/L: 62.49±6.66, 48.39±2.37 vs. 10.75±0.74, both P < 0.05), and peaked at 2 hours (pg/L: 70.93±5.51, 66.03±2.60 vs. 10.87±0.91, both P < 0.05) followed by a gradual decline. The levels of serum IL-6 at 0.5 hours after ROSC in CA-CPR 12 hours and 24 hours groups were markedly higher than those of sham group (pg/L: 14.42±1.99, 11.23±1.12 vs. 8.75±0.74, both P < 0.05), and peaked at 12 hours (pg/L: 36.50±2.91, 38.15±1.26 vs. 8.88±0.62, both P < 0.05) followed by a gradual decline. The protein expressions of TLR4 and NF-κB in the myocardium were significantly increased in CA-CPR 12 hours and 24 hours groups as compared with sham group [TLR4 protein (gray value): 0.11±0.03, 0.24±0.05 vs. 0.05±0.02; NF-κB protein (gray value): 0.27±0.04, 0.24±0.03 vs. 0.09±0.02, all P < 0.05]. The mRNA levels of TLR4 in CA-CPR 12 hours and 24 hours groups were increased by approximately (9.93±1.07) folds and (9.21±1.27) folds of sham group respectively, and NF-κB mRNA expressions were increased by (4.44±0.96) folds and (6.09±0.81) folds of sham group respectively (all P < 0.01).ConclusionActivation of TLR4/NF-κB signal pathway may be one of the main pathological mechanisms of post resuscitation myocardial injury in a porcine model of CA-CPR.Key words: Cardiopulmonary resuscitation; Myocardial dysfunction; Toll like receptor 4/nuclear factor-κB signal pathway; Interleukin-6; Tumor necrosis factor-α" @default.
• W3032795231 created "2020-06-05" @default.
• W3032795231 creator A5017452803 @default.
• W3032795231 creator A5024542921 @default.
• W3032795231 creator A5028628100 @default.
• W3032795231 creator A5075937517 @default.
• W3032795231 date "2016-07-10" @default.
• W3032795231 modified "2023-09-26" @default.
• W3032795231 title "Role of Toll like receptor 4/nuclear factor-κB signal pathway on the pathogenesis of acute myocardial dysfunction after cardiopulmonary resuscitation in pigs" @default.
• W3032795231 doi "https://doi.org/10.3760/cma.j.issn.2095-4352.2016.07.003" @default.
• W3032795231 hasPublicationYear "2016" @default.
• W3032795231 type Work @default.
• W3032795231 sameAs 3032795231 @default.
• W3032795231 citedByCount "0" @default.
• W3032795231 crossrefType "journal-article" @default.
• W3032795231 hasAuthorship W3032795231A5017452803 @default.
• W3032795231 hasAuthorship W3032795231A5024542921 @default.
• W3032795231 hasAuthorship W3032795231A5028628100 @default.
• W3032795231 hasAuthorship W3032795231A5075937517 @default.
• W3032795231 hasConcept C126322002 @default.
• W3032795231 hasConcept C134018914 @default.
• W3032795231 hasConcept C164705383 @default.
• W3032795231 hasConcept C170493617 @default.
• W3032795231 hasConcept C178853913 @default.
• W3032795231 hasConcept C17991360 @default.
• W3032795231 hasConcept C2776070231 @default.
• W3032795231 hasConcept C2777055891 @default.
• W3032795231 hasConcept C2778165595 @default.
• W3032795231 hasConcept C2778619729 @default.
• W3032795231 hasConcept C2780942790 @default.
• W3032795231 hasConcept C42219234 @default.
• W3032795231 hasConcept C71924100 @default.
• W3032795231 hasConceptScore W3032795231C126322002 @default.
• W3032795231 hasConceptScore W3032795231C134018914 @default.
• W3032795231 hasConceptScore W3032795231C164705383 @default.
• W3032795231 hasConceptScore W3032795231C170493617 @default.
• W3032795231 hasConceptScore W3032795231C178853913 @default.
• W3032795231 hasConceptScore W3032795231C17991360 @default.
• W3032795231 hasConceptScore W3032795231C2776070231 @default.
• W3032795231 hasConceptScore W3032795231C2777055891 @default.
• W3032795231 hasConceptScore W3032795231C2778165595 @default.
• W3032795231 hasConceptScore W3032795231C2778619729 @default.
• W3032795231 hasConceptScore W3032795231C2780942790 @default.
• W3032795231 hasConceptScore W3032795231C42219234 @default.
• W3032795231 hasConceptScore W3032795231C71924100 @default.
• W3032795231 hasIssue "7" @default.
• W3032795231 hasLocation W30327952311 @default.
• W3032795231 hasOpenAccess W3032795231 @default.
• W3032795231 hasPrimaryLocation W30327952311 @default.
• W3032795231 hasRelatedWork W161399153 @default.
• W3032795231 hasRelatedWork W2375087367 @default.
• W3032795231 hasRelatedWork W2379051265 @default.
• W3032795231 hasRelatedWork W2409728628 @default.
• W3032795231 hasRelatedWork W2418706190 @default.
• W3032795231 hasRelatedWork W2461322061 @default.
• W3032795231 hasRelatedWork W2915517768 @default.
• W3032795231 hasRelatedWork W2981289522 @default.
• W3032795231 hasRelatedWork W3006077856 @default.
• W3032795231 hasRelatedWork W3026041626 @default.
• W3032795231 hasRelatedWork W3029049688 @default.
• W3032795231 hasRelatedWork W3029189729 @default.
• W3032795231 hasRelatedWork W3029317681 @default.
• W3032795231 hasRelatedWork W3029622112 @default.
• W3032795231 hasRelatedWork W3031086769 @default.
• W3032795231 hasRelatedWork W3031154194 @default.
• W3032795231 hasRelatedWork W3031309781 @default.
• W3032795231 hasRelatedWork W3031638385 @default.
• W3032795231 hasRelatedWork W3032137321 @default.
• W3032795231 hasRelatedWork W3088419516 @default.
• W3032795231 hasVolume "28" @default.
• W3032795231 isParatext "false" @default.
• W3032795231 isRetracted "false" @default.
• W3032795231 magId "3032795231" @default.
• W3032795231 workType "article" @default.