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- W3032799139 abstract "Objective To explore the conditions and efficiency of YFP-labeled mouse hematopoietic stem and progenitor cells induced by ERT2-Cre.Methods ERT2-Cre and YFP double-positive transgenic hybrid mice from all offsprings of Rosa-YFP mice and ERT2-Cre mice were obtained by genotyping,and c-kit+ cells were enriched in vitro using magnetic activated cell sorting from bone marrow cells of double-positive transgenic hybrid mice.The expression of YFP was induced by different concentrations of 4-hydroxytamoxifen (OHT) with different periods,and the expressing efficiency of YFP in c-kit+ cells was detected by flow cytometry.Results Five out of nine Rosa26R-YFP and ERT2-Cre mice crossbred progenies were YFP and Cre transgene double positive.Some morphological changes of c-kit+ cells cultured in vitro suggested the presence of cell differentiation.The expressing efficiency of YFP in c-kit + cells was up to 13.7％.Conclusions YFP reporter gene system can effectively mark mouse hematopoietic stem and progenitor cells induced by ERT2-Cre with the exist of OHT in vitro.Key words: Hematopoietic system; Hematopoietic stem and progenitor cells; c-kit+ cells; ERT2-Cre; Rosa26R-YFP reporter gene system" @default.
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- W3032799139 date "2014-10-28" @default.
- W3032799139 modified "2023-09-23" @default.
- W3032799139 title "Conditions and efficiency of Rosa26R-YFP reporter gene system labeled mouse hematopoietic stem and progenitor cells induced by ERT2-Cre" @default.
- W3032799139 doi "https://doi.org/10.3760/cma.j.issn.1673-4181.2014.05.003" @default.
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