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• W3032839742 abstract "ObjectiveTo investigate the characteristic parameters and variation of acoustic startle reflex (ASR) and conditioning response(CR)during the acquisition of delay-trace eyeblink conditioning.Methods15 healthy Kunming mice were randomly divided into 3 groups: single-task DEBC group (n=5); single-task TEBC group (n=5); dual delay/trace group (n=5). Three groups received paired training of tone conditioned stimulus(CS)in different audio frequency ( TEBC groups: 2KHz; DEBC group: 1KHz) and a 100 ms comeal oxygen-puff unconditioned stimulus(US). Then observed the characteristic parameters of ASR and CR.ResultsAfter pairing training for 10 days, ASR rate(ASR%) of single-task groups (10th day (38.00±8.64)% and (38.55±12.41)% respectively, time effect: F=4.574, P=0.008; group effect: F=2.021, P=0.193) and dual delay-trace group (10th day (47.95±14.23)% and (62.01±9.03)% respectively, time effect: F=5, 547 P=0.013; group effect: F=0.738, P=0.415) changed significantly with the following training but between single-task groups or dual delay-trace group. CR rate(CR%) of single-task groups (10th day were (45.4±5.39)% and (65.6±6.77)% respectively, time effect: F=9.558, P=0.000; group effect: F=5.117, P=0.054) and dual delay/trace group (10th day (57.66±4.34)% and (77.35±7.36)% respectively, time effect: F=7.750, P=0.002; group effect: F=1.449, P=0.263) showed obvious change with the following training but between single-task groups or dual delay-trace group.Peak amplitude of single-task DEBC group(time effect F=2.679, P=0.017)and dual delay-trace group (F=3.452, P=0.034)had increased significantly with the following training.Peak latency showed no obvious change.ConclusionDual delay-trace conditioning procedure of classical eyeblink conditioning and single-task can be successfully established in Kunming mice .Although there are some variations, those can be controled and be weaken effect of them by taking targeted measures.Key words: Dual delay-trace conditioning procedure; Eyeblink conditioning; Acoustic startle reflex; Mice" @default.
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• W3032839742 date "2012-08-20" @default.
• W3032839742 modified "2023-09-28" @default.
• W3032839742 title "Establishment of delay-trace conditioning procedure of classical eyeblink conditioning in Kunming mice and analysis of behavioral characteristic parameters" @default.
• W3032839742 doi "https://doi.org/10.3760/cma.j.issn.1674-6554.2012.08.001" @default.
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