FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3032978562> ?p ?o ?g. }

• W3032978562 endingPage "11179" @default.
• W3032978562 startingPage "11170" @default.
• W3032978562 abstract "The development of artificial proteases is challenging, but important for many applications in modern proteomics and biotechnology. The hydrolysis of hydrophobic or unstructured proteins is particularly difficult due to their poor solubility, which often requires the presence of surfactants. Herein, it is shown that a zirconium(IV)-substituted Keggin polyoxometalate (POM), (Et2NH2)10[Zr(α-PW11O39)2] (1), is able to selectively hydrolyze β-casein, which is an intrinsically unstructured protein at pH 7.4 and 60 °C. Four surfactants (sodium dodecyl sulfate (SDS), N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (ZW3-12), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), and polyethylene glycol tert-octylphenyl ether (TX-100)), which differ in the nature of their polar groups, were investigated for their role in influencing the selectivity and efficiency of protein hydrolysis. Under experimental conditions, β-casein forms micellar structures in which the hydrophilic part of the protein is water accessible and able to interact with 1. Identical fragmentation patterns of β-casein in the presence of 1 were observed through SDS poly(acrylamide) gel electrophoresis both in the presence and absence of surfactants, but the rate of hydrolysis varied, depending on the nature of surfactant. Whereas TX-100 surfactant, which has a neutral polar head, caused only a slight decrease in the hydrolysis rate, stronger inhibition was observed in the presence surfactants with charges in their polar heads (CHAPS, ZW3-12, SDS). These results were consistent with those of tryptophan fluorescencequenching studies, which showed that the binding between β-casein and 1 decreased with increasing repulsion between the POM and the polar heads of the surfactants. In all cases, the micellar structure of β-casein was not significantly affected by the presence of POM or surfactants, as indicated by circular dichroism spectroscopy." @default.
• W3032978562 created "2020-06-12" @default.
• W3032978562 creator A5030890226 @default.
• W3032978562 creator A5058335921 @default.
• W3032978562 creator A5088887597 @default.
• W3032978562 date "2020-08-13" @default.
• W3032978562 modified "2023-09-29" @default.
• W3032978562 title "Hydrolysis of Peptide Bonds in Protein Micelles Promoted by a Zirconium(IV)‐Substituted Polyoxometalate as an Artificial Protease" @default.
• W3032978562 cites W1143768377 @default.
• W3032978562 cites W1175056953 @default.
• W3032978562 cites W1571315859 @default.
• W3032978562 cites W1602375806 @default.
• W3032978562 cites W1622131884 @default.
• W3032978562 cites W1968031065 @default.
• W3032978562 cites W1970956560 @default.
• W3032978562 cites W1989270637 @default.
• W3032978562 cites W1991127420 @default.
• W3032978562 cites W1991800717 @default.
• W3032978562 cites W2010013989 @default.
• W3032978562 cites W2016099897 @default.
• W3032978562 cites W2016170088 @default.
• W3032978562 cites W2017557420 @default.
• W3032978562 cites W2024211300 @default.
• W3032978562 cites W2026727321 @default.
• W3032978562 cites W2034140489 @default.
• W3032978562 cites W2035127456 @default.
• W3032978562 cites W2039446118 @default.
• W3032978562 cites W2046482444 @default.
• W3032978562 cites W2047182006 @default.
• W3032978562 cites W2049315128 @default.
• W3032978562 cites W2059726111 @default.
• W3032978562 cites W2063060157 @default.
• W3032978562 cites W2063249346 @default.
• W3032978562 cites W2063807188 @default.
• W3032978562 cites W2064503260 @default.
• W3032978562 cites W2075076588 @default.
• W3032978562 cites W2075129446 @default.
• W3032978562 cites W2075849880 @default.
• W3032978562 cites W2075943744 @default.
• W3032978562 cites W2077347443 @default.
• W3032978562 cites W2077428368 @default.
• W3032978562 cites W2078040293 @default.
• W3032978562 cites W2080489533 @default.
• W3032978562 cites W2083844888 @default.
• W3032978562 cites W2088474620 @default.
• W3032978562 cites W2091607049 @default.
• W3032978562 cites W2100710272 @default.
• W3032978562 cites W2101713007 @default.
• W3032978562 cites W2106111156 @default.
• W3032978562 cites W2116115838 @default.
• W3032978562 cites W2128172145 @default.
• W3032978562 cites W2140260269 @default.
• W3032978562 cites W2142219912 @default.
• W3032978562 cites W2142830992 @default.
• W3032978562 cites W2146493759 @default.
• W3032978562 cites W2324422030 @default.
• W3032978562 cites W2334665661 @default.
• W3032978562 cites W2336043143 @default.
• W3032978562 cites W2491458126 @default.
• W3032978562 cites W2517327512 @default.
• W3032978562 cites W2537043041 @default.
• W3032978562 cites W2607500394 @default.
• W3032978562 cites W2612247019 @default.
• W3032978562 cites W2724716997 @default.
• W3032978562 cites W2768414976 @default.
• W3032978562 cites W2777004883 @default.
• W3032978562 cites W2781249179 @default.
• W3032978562 cites W2782932396 @default.
• W3032978562 cites W2790706059 @default.
• W3032978562 cites W2804344089 @default.
• W3032978562 cites W2832524598 @default.
• W3032978562 cites W2889436585 @default.
• W3032978562 cites W2893559110 @default.
• W3032978562 cites W2903321785 @default.
• W3032978562 cites W2904533352 @default.
• W3032978562 cites W2907384381 @default.
• W3032978562 cites W2912126500 @default.
• W3032978562 cites W2949843201 @default.
• W3032978562 cites W2950549505 @default.
• W3032978562 cites W2952979599 @default.
• W3032978562 cites W2970427885 @default.
• W3032978562 cites W2977715662 @default.
• W3032978562 cites W2981235867 @default.
• W3032978562 cites W3009002977 @default.
• W3032978562 cites W4232185861 @default.
• W3032978562 cites W4361867824 @default.
• W3032978562 doi "https://doi.org/10.1002/chem.202001920" @default.
• W3032978562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32515831" @default.
• W3032978562 hasPublicationYear "2020" @default.
• W3032978562 type Work @default.
• W3032978562 sameAs 3032978562 @default.
• W3032978562 citedByCount "11" @default.
• W3032978562 countsByYear W30329785622021 @default.
• W3032978562 countsByYear W30329785622022 @default.
• W3032978562 countsByYear W30329785622023 @default.
• W3032978562 crossrefType "journal-article" @default.
• W3032978562 hasAuthorship W3032978562A5030890226 @default.
• W3032978562 hasAuthorship W3032978562A5058335921 @default.

• next