FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3033271402> ?p ?o ?g. }

• W3033271402 endingPage "e0232610" @default.
• W3033271402 startingPage "e0232610" @default.
• W3033271402 abstract "Pneumonia is one of the most important causes of morbidity and mortality in children. Identification and characterization of pathogens that cause infections are crucial for accurate treatment and accelerated recovery. However, in most cases, the causative agent cannot be identified, which is partly due to the limited spectrum of pathogens covered by current diagnostics based on nucleic acid amplification. Therefore, in this study, we explored the application of metagenomic next-generation sequencing (mNGS) for the diagnosis of children with severe pneumonia. From April to July 2017, 32 hospitalized children with severe nonresponding pneumonia in Shenzhen Children’s Hospital were included in this study. Blood tests were conducted immediately after hospitalization to assess cell counts and inflammatory markers, oropharyngeal swabs were collected to identify common pathogens by qPCR and culture. After bronchoscopy, bronchoalveolar lavage fluid (BALF) samples were collected for further pathogen identification using standardized diagnostic tests and mNGS. Blood tests were normal in 3 of the 32 children. In 9 oropharyngeal swabs, bacterial pathogens were detected, in 5 of these Mycoplasma pneumoniae was detected. Adenovirus was detected in 5 BALF samples, using the Direct Immunofluorescence Assay (DFA). In 15 cases, no common pathogens were found in BALF samples, using the current standard diagnostic tests, while in all 32 BALFs, pathogens were identified using mNGS, including adenovirus, Mycoplasma pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, cytomegalovirus and bocavirus. This study shows that, with mNGS, the sensitivity of detection of the causative pathogens in children with severe nonresponding pneumonia is significantly improved. In addition, mNGS gives more strain specific information, helps to identify new pathogens and could potentially help to trace and control outbreaks. In this study, we have shown that it is possible to have the results within 24 hours, making the application of mNGS feasible for clinical diagnostics." @default.
• W3033271402 created "2020-06-12" @default.
• W3033271402 creator A5005347732 @default.
• W3033271402 creator A5012525675 @default.
• W3033271402 creator A5015324472 @default.
• W3033271402 creator A5023909153 @default.
• W3033271402 creator A5025322442 @default.
• W3033271402 creator A5028172643 @default.
• W3033271402 creator A5032417925 @default.
• W3033271402 creator A5068063896 @default.
• W3033271402 date "2020-06-04" @default.
• W3033271402 modified "2023-10-18" @default.
• W3033271402 title "Clinical diagnostic application of metagenomic next-generation sequencing in children with severe nonresponding pneumonia" @default.
• W3033271402 cites W1980460814 @default.
• W3033271402 cites W2000714505 @default.
• W3033271402 cites W2048107714 @default.
• W3033271402 cites W2099819970 @default.
• W3033271402 cites W2106780559 @default.
• W3033271402 cites W2107315130 @default.
• W3033271402 cites W2136114024 @default.
• W3033271402 cites W2150069228 @default.
• W3033271402 cites W2188044678 @default.
• W3033271402 cites W2332382218 @default.
• W3033271402 cites W2493434520 @default.
• W3033271402 cites W2513861476 @default.
• W3033271402 cites W2587565773 @default.
• W3033271402 cites W2611595334 @default.
• W3033271402 cites W2726960688 @default.
• W3033271402 cites W2736085563 @default.
• W3033271402 cites W2771296805 @default.
• W3033271402 cites W2886760636 @default.
• W3033271402 cites W2887244782 @default.
• W3033271402 cites W4239909494 @default.
• W3033271402 doi "https://doi.org/10.1371/journal.pone.0232610" @default.
• W3033271402 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7272011" @default.
• W3033271402 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32497137" @default.
• W3033271402 hasPublicationYear "2020" @default.
• W3033271402 type Work @default.
• W3033271402 sameAs 3033271402 @default.
• W3033271402 citedByCount "32" @default.
• W3033271402 countsByYear W30332714022020 @default.
• W3033271402 countsByYear W30332714022021 @default.
• W3033271402 countsByYear W30332714022022 @default.
• W3033271402 countsByYear W30332714022023 @default.
• W3033271402 crossrefType "journal-article" @default.
• W3033271402 hasAuthorship W3033271402A5005347732 @default.
• W3033271402 hasAuthorship W3033271402A5012525675 @default.
• W3033271402 hasAuthorship W3033271402A5015324472 @default.
• W3033271402 hasAuthorship W3033271402A5023909153 @default.
• W3033271402 hasAuthorship W3033271402A5025322442 @default.
• W3033271402 hasAuthorship W3033271402A5028172643 @default.
• W3033271402 hasAuthorship W3033271402A5032417925 @default.
• W3033271402 hasAuthorship W3033271402A5068063896 @default.
• W3033271402 hasBestOaLocation W30332714021 @default.
• W3033271402 hasConcept C126322002 @default.
• W3033271402 hasConcept C159047783 @default.
• W3033271402 hasConcept C203014093 @default.
• W3033271402 hasConcept C2776012195 @default.
• W3033271402 hasConcept C2776460866 @default.
• W3033271402 hasConcept C2776551000 @default.
• W3033271402 hasConcept C2776925733 @default.
• W3033271402 hasConcept C2777714996 @default.
• W3033271402 hasConcept C2777719358 @default.
• W3033271402 hasConcept C2777914695 @default.
• W3033271402 hasConcept C2777961210 @default.
• W3033271402 hasConcept C2779215961 @default.
• W3033271402 hasConcept C2781253189 @default.
• W3033271402 hasConcept C501593827 @default.
• W3033271402 hasConcept C534529494 @default.
• W3033271402 hasConcept C71924100 @default.
• W3033271402 hasConcept C86803240 @default.
• W3033271402 hasConcept C89423630 @default.
• W3033271402 hasConceptScore W3033271402C126322002 @default.
• W3033271402 hasConceptScore W3033271402C159047783 @default.
• W3033271402 hasConceptScore W3033271402C203014093 @default.
• W3033271402 hasConceptScore W3033271402C2776012195 @default.
• W3033271402 hasConceptScore W3033271402C2776460866 @default.
• W3033271402 hasConceptScore W3033271402C2776551000 @default.
• W3033271402 hasConceptScore W3033271402C2776925733 @default.
• W3033271402 hasConceptScore W3033271402C2777714996 @default.
• W3033271402 hasConceptScore W3033271402C2777719358 @default.
• W3033271402 hasConceptScore W3033271402C2777914695 @default.
• W3033271402 hasConceptScore W3033271402C2777961210 @default.
• W3033271402 hasConceptScore W3033271402C2779215961 @default.
• W3033271402 hasConceptScore W3033271402C2781253189 @default.
• W3033271402 hasConceptScore W3033271402C501593827 @default.
• W3033271402 hasConceptScore W3033271402C534529494 @default.
• W3033271402 hasConceptScore W3033271402C71924100 @default.
• W3033271402 hasConceptScore W3033271402C86803240 @default.
• W3033271402 hasConceptScore W3033271402C89423630 @default.
• W3033271402 hasFunder F4320335767 @default.
• W3033271402 hasFunder F4320335966 @default.
• W3033271402 hasIssue "6" @default.
• W3033271402 hasLocation W30332714021 @default.
• W3033271402 hasLocation W30332714022 @default.
• W3033271402 hasLocation W30332714023 @default.
• W3033271402 hasLocation W30332714024 @default.
• W3033271402 hasLocation W30332714025 @default.

• next