FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3033356804> ?p ?o ?g. }

• W3033356804 abstract "Abstract Background and Aims Drug-induced acute kidney injury (AKI) is a health problem that increases morbidity and mortality rates as well as costs to health systems. Amphotericin B (AnB) is a widely used antifungal, which its therapy is closely associated with nephrotoxicity. The mechanisms of nephrotoxicity are not yet fully understood and there is a need to search for substances that prevent and / or reverse the associated renal damage. (-) - α-Bisabolol (BIS) has been demonstrated nephroprotective effect due to its antioxidant action. Thus, the aim of this study was to evaluated the effect of BIS as a protective substance on renal cell injury induced by amphotericin B. Method LLC-MK2 tubular renal cells were treated with different concentrations of BIS and AnB and its viability was measured by MTT assay to define the working concentrations of BIS and AnB. Then, cells were pretreated or post treated with BIS (31.25 - 125 μM) and AnB (13.97 μM) for 24 hours to evaluate the protective effect of BIS. Control (CT) was treated with sterile PBS. The experimental groups were further submitted to flow cytometry assays for membrane integrity evaluation using 7-AAD and measurement of cytoplasmic ROS production using DCFH-DA. The experiments were performed in triplicate (n = 3), and the data analyzed by One-Way ANOVA with Bonferroni post-test. Results BIS concentrations from 31.25 to 125 μM were selected, as well as the IC50 for AnB (13.97 ± 1.45 μM) for the furthers assays. Pretreatment with BIS increased cell viability by about 10% (31.25 – 63.31 ± 2.04%; 62.5 – 64.75 ± 2.10%; 125 – 63.92 ± 2.72%) compared to the AnB-only group (49.79 ± 1.25%), which had its cell viability reduced by about 50% (CT – 100.70 ± 0.83%). BIS was also able to increase the whole cell population at concentrations of 62.5 (83.10 ± 1.10%) and 125 μM (84.67 ± 0.98%) when compared to the AnB group (76.54 ± 1.18%). In DCF labeling, it was observed that AnB increased the production of ROS in 20% (IFR = 1.21 ± 0.02) when compared to the control (IFR = 1.00 ± 0.01), and BIS (31.25 μM) was able to reduce in 41% (IFR = 0.79 ± 0.01) when compared to the AnB group. Conclusion The (-)-α-bisabolol was able to prevent the renal cytotoxicity induced by amphotericin." @default.
• W3033356804 created "2020-06-12" @default.
• W3033356804 creator A5012039671 @default.
• W3033356804 creator A5025801362 @default.
• W3033356804 creator A5056202905 @default.
• W3033356804 creator A5058993846 @default.
• W3033356804 creator A5072393110 @default.
• W3033356804 creator A5077582965 @default.
• W3033356804 creator A5082530880 @default.
• W3033356804 creator A5088912194 @default.
• W3033356804 date "2020-06-01" @default.
• W3033356804 modified "2023-09-26" @default.
• W3033356804 title "P0553PROTECTIVE EFFECT OF (-)-ALPHA-BISABOLOL OVER RENAL CYTOTOXICITY INDUCED BY AMPHOTERICIN B" @default.
• W3033356804 doi "https://doi.org/10.1093/ndt/gfaa142.p0553" @default.
• W3033356804 hasPublicationYear "2020" @default.
• W3033356804 type Work @default.
• W3033356804 sameAs 3033356804 @default.
• W3033356804 citedByCount "0" @default.
• W3033356804 crossrefType "journal-article" @default.
• W3033356804 hasAuthorship W3033356804A5012039671 @default.
• W3033356804 hasAuthorship W3033356804A5025801362 @default.
• W3033356804 hasAuthorship W3033356804A5056202905 @default.
• W3033356804 hasAuthorship W3033356804A5058993846 @default.
• W3033356804 hasAuthorship W3033356804A5072393110 @default.
• W3033356804 hasAuthorship W3033356804A5077582965 @default.
• W3033356804 hasAuthorship W3033356804A5082530880 @default.
• W3033356804 hasAuthorship W3033356804A5088912194 @default.
• W3033356804 hasConcept C109316439 @default.
• W3033356804 hasConcept C126189478 @default.
• W3033356804 hasConcept C126322002 @default.
• W3033356804 hasConcept C1491633281 @default.
• W3033356804 hasConcept C16005928 @default.
• W3033356804 hasConcept C185592680 @default.
• W3033356804 hasConcept C202751555 @default.
• W3033356804 hasConcept C2779548794 @default.
• W3033356804 hasConcept C2779629538 @default.
• W3033356804 hasConcept C2780091579 @default.
• W3033356804 hasConcept C53227056 @default.
• W3033356804 hasConcept C55493867 @default.
• W3033356804 hasConcept C71924100 @default.
• W3033356804 hasConcept C98274493 @default.
• W3033356804 hasConceptScore W3033356804C109316439 @default.
• W3033356804 hasConceptScore W3033356804C126189478 @default.
• W3033356804 hasConceptScore W3033356804C126322002 @default.
• W3033356804 hasConceptScore W3033356804C1491633281 @default.
• W3033356804 hasConceptScore W3033356804C16005928 @default.
• W3033356804 hasConceptScore W3033356804C185592680 @default.
• W3033356804 hasConceptScore W3033356804C202751555 @default.
• W3033356804 hasConceptScore W3033356804C2779548794 @default.
• W3033356804 hasConceptScore W3033356804C2779629538 @default.
• W3033356804 hasConceptScore W3033356804C2780091579 @default.
• W3033356804 hasConceptScore W3033356804C53227056 @default.
• W3033356804 hasConceptScore W3033356804C55493867 @default.
• W3033356804 hasConceptScore W3033356804C71924100 @default.
• W3033356804 hasConceptScore W3033356804C98274493 @default.
• W3033356804 hasLocation W30333568041 @default.
• W3033356804 hasOpenAccess W3033356804 @default.
• W3033356804 hasPrimaryLocation W30333568041 @default.
• W3033356804 hasRelatedWork W1362068 @default.
• W3033356804 hasRelatedWork W15246896 @default.
• W3033356804 hasRelatedWork W18227388 @default.
• W3033356804 hasRelatedWork W20420110 @default.
• W3033356804 hasRelatedWork W2434354 @default.
• W3033356804 hasRelatedWork W5027642 @default.
• W3033356804 hasRelatedWork W6720303 @default.
• W3033356804 hasRelatedWork W7534746 @default.
• W3033356804 hasRelatedWork W10172238 @default.
• W3033356804 hasRelatedWork W5087632 @default.
• W3033356804 isParatext "false" @default.
• W3033356804 isRetracted "false" @default.
• W3033356804 magId "3033356804" @default.
• W3033356804 workType "article" @default.