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- W3033382009 abstract "Abstract Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (Re L ) and the k L a coefficient, as key parameters characterized the bioprocessing of HL-60 cells in ReadyToProcess WAVE TM 25 system, on reached values of the apparent maximal specific growth rate ( μ max ) and the specific yield of biomass ( Y * X/S ) has been identified. The values of Re L (i.e., 510–10,208), as well as k L a coefficient (i.e., 2.83–13.55 h −1 ), have been estimated for the cultures subjected to wave-induced mixing, based on simplified dimensionless correlation for various presents of WAVE 25 system. The highest values of apparent μ max = 0.038 h −1 and Y * X/S = 25.64 × 10 8 cells g glc −1 have been noted for cultures independently performed at wave-induced agitation characterized by Re L equaled to 5104 and 510, respectively. The presented results have high applicability potential in scale-up of bioprocesses focused on nonadherent animal cells, or in the case of any application of disposable bioreactors presenting similitude." @default.
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- W3033382009 date "2020-06-09" @default.
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- W3033382009 title "Efficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number" @default.
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- W3033382009 doi "https://doi.org/10.1007/s00449-020-02386-6" @default.
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