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- W3033631418 abstract "Purpose This study was conducted to assess the anticancer role of gammacerane against human endometrial cancer. Methods The human RL-95 cell line (endometrial cancer) and SV40 (normal endometrial cells) were used in this study. The MTT-based estimation of cell proliferation assay along with the colony formation assay were used for assessing the cell viability. Acridine orange (AO)/Ethidium bromide (EB) staining followed by fluorescent microscopy was performed for estimation of cell apoptosis. Flow cytometry was used to assess the cell cycle phase distribution of cancer cells. Cell migration and invasion were estimated using wound healing and transwell assay, respectively. Western blotting was used for protein expression studies. Results The cell proliferation assay revealed that gammacerane treatment led to loss of viability of RL-95 cancer cells in a concentration-dependent manner. However, the antiproliferative effects were comparatively less prominent when gammacerane was used against the SV40 normal endometrial cells. AO/EB staining of cancer cells showed that gammacerane is active in inducing apoptosis in RL-95 cells and apoptotic induction effects were more evident at higher concentrations of the molecule. Flow cytometric analysis with Annexin V-FITC/Propidium iodide (PI) fixed cells showed that the percentage of apoptotic cells increased with increase in gammacerane concentration. Apoptotic signal was mediated via the modulation of Bax/Bcl-2 protein ratio. Western blot analysis of STAT3 protein showed that gammacerane treatment reduced the protein levels of STAT3 and the effects were more prominent at higher treatment concentrations. Conclusion Gammacerane, by its ability to take control over the transcription of STAT3 transcription factor, inhibits the proliferation of human endometrial cancer cells. The effects revealed loss of viability, arrest of mitosis and cellular apoptosis." @default.
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- W3033631418 date "2020-03-01" @default.
- W3033631418 modified "2023-09-28" @default.
- W3033631418 title "Gammacerane suppresses the growth of human endometrial carcinoma cells via induction of apoptosis, cell cycle arrest, inhibition of cell migration, invasion and STAT3 signaling pathway." @default.
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