FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3033654687> ?p ?o ?g. }

• W3033654687 endingPage "455" @default.
• W3033654687 startingPage "454.2" @default.
• W3033654687 abstract "Background: Chondrocalcinosis is a painful rheumatic condition caused by the deposition of calcium pyrophosphate dihydrate crystals (CPPD) in joint tissues, and especially in cartilage. It is known that CPPD crystals cause inflammation and degenerative changes in joint, but the underlying mechanisms remain poorly understood. In particular, nothing is known about how these crystals regulates transmembrane heparan sulphate proteoglycans (HSPGs). Our attention focused on one family of HSPGs called syndecans as they have important roles both as adhesion molecules, by mediating chondrocyte-extracellular matrix interactions, and as modulators of intracellular signaling triggered by cytokines and growth factors. Objectives: The aim of this study was to evaluate how CPPD crystals modulates syndecan expression in chondrocytes and in cartilage, and how this modulation can be ultimately linked to cartilage damage during chondrocylcinosis. Methods: Murine chondrocitic ATDC5 cells were stimulated with 0,1 ng/ml CPPD crystals or with 0,1 ng/ml basic-calcium phosphate crystals (BCP), a family of calcium-containing crystals found in other rheumatic conditions such as osteoarthritis (OA). Cytotoxicity was evaluated by lactate dehydrogenase (LDH) release in the supernatant at 30 minutes, and 3, 6, 24 hours after stimulation. At the same time-points, mRNA expression levels of syndecans (Synd-1, -2, -3, -4) and of matrix-degrading enzymes (Mmp-3, -9, -13; Adamts-4, -5) was analysed by qRT-PCR. Finally, Syndecan-4 protein expression was studied by immunohistochemistry (IHC) in cartilage samples of patients with chondrocalcinosis and in samples of patients with severe OA without chondrocalcinosis as control. Results: LDH assay revealed no increased cytotoxicity by CPPD or BCP at any time-point. qRT-PCR indicated that CPPD crystals but not BCP crystals induced Synd-2 and -3 upregulation at 30 minutes after stimulation and Synd-4 upregulation at 3 hours, while no modulation of syndecans was seen at later time-points. CPPD also induced Adamts-4 expression at 3 hours after stimulation, and Mmp-9 expression at 3 and 6 hours. The expression of the other matrix-degrading enzymes was not affected. Human chondrocalcinosis cartilage exhibited enhanced Synd-4 expression compared to severe OA cartilage containing BCP calcification. Interestingly, Synd-4 expression was observed in the extracellular matrix but not on cell membrane, suggesting that maybe Synd-4 undergoes shedding (Figure 1). Figure 1. Representative Synd-4 IHC in control patients (Ctrl, severe OA) and chondrocalcinosis patients (CPPD). Note increased Synd-4 expression in extracellular matrix of CPPD patients compared to Ctrl ones. Conclusion: BCP and CPPD crystals seem to trigger differential effects in terms of modulation of syndecans in chondrocitic cells. CPPD crystals induce Synd-4 and Adamts-4 and Mmp-9 which are not induced by BCP crystals. It remains to be clarified whether the two events are interlinked. In particular, further studies are required to investigate if Adamts-4 and Mmp-9 are involved in Synd-4 shedding or if vice versa Synd-4 regulates Adamts-4 and Mmp-9 activation and downstream cartilage breakdown in chondrocalcinosis. Disclosure of Interests: : Sonia Nasi: None declared, Jessica Bertrand Grant/research support from: Pfizer, Speakers bureau: Pfizer, Miriam Bollmann: None declared, Richard Stange: None declared, Thomas Pap: None declared" @default.
• W3033654687 created "2020-06-12" @default.
• W3033654687 creator A5008979776 @default.
• W3033654687 creator A5011049345 @default.
• W3033654687 creator A5026582019 @default.
• W3033654687 creator A5028798083 @default.
• W3033654687 creator A5074330931 @default.
• W3033654687 date "2020-06-01" @default.
• W3033654687 modified "2023-09-25" @default.
• W3033654687 title "THU0435 CALCIUM PYROPHOSPHATE DIHYDRATE (CPPD) CRYSTALS BUT NOT BASIC CALCIUM PHOSPHATE (BCP) CRYSTALS INDUCE SYNDECAN-4 EXPRESSION IN CARTILAGE" @default.
• W3033654687 doi "https://doi.org/10.1136/annrheumdis-2020-eular.2772" @default.
• W3033654687 hasPublicationYear "2020" @default.
• W3033654687 type Work @default.
• W3033654687 sameAs 3033654687 @default.
• W3033654687 citedByCount "0" @default.
• W3033654687 crossrefType "journal-article" @default.
• W3033654687 hasAuthorship W3033654687A5008979776 @default.
• W3033654687 hasAuthorship W3033654687A5011049345 @default.
• W3033654687 hasAuthorship W3033654687A5026582019 @default.
• W3033654687 hasAuthorship W3033654687A5028798083 @default.
• W3033654687 hasAuthorship W3033654687A5074330931 @default.
• W3033654687 hasBestOaLocation W30336546871 @default.
• W3033654687 hasConcept C105702510 @default.
• W3033654687 hasConcept C126322002 @default.
• W3033654687 hasConcept C142724271 @default.
• W3033654687 hasConcept C1491633281 @default.
• W3033654687 hasConcept C153074725 @default.
• W3033654687 hasConcept C153911025 @default.
• W3033654687 hasConcept C181080969 @default.
• W3033654687 hasConcept C185592680 @default.
• W3033654687 hasConcept C189165786 @default.
• W3033654687 hasConcept C204787440 @default.
• W3033654687 hasConcept C2776164576 @default.
• W3033654687 hasConcept C2778155236 @default.
• W3033654687 hasConcept C2779335624 @default.
• W3033654687 hasConcept C2780550940 @default.
• W3033654687 hasConcept C2780608848 @default.
• W3033654687 hasConcept C2781403057 @default.
• W3033654687 hasConcept C519063684 @default.
• W3033654687 hasConcept C55493867 @default.
• W3033654687 hasConcept C65001120 @default.
• W3033654687 hasConcept C71924100 @default.
• W3033654687 hasConcept C86803240 @default.
• W3033654687 hasConcept C95444343 @default.
• W3033654687 hasConceptScore W3033654687C105702510 @default.
• W3033654687 hasConceptScore W3033654687C126322002 @default.
• W3033654687 hasConceptScore W3033654687C142724271 @default.
• W3033654687 hasConceptScore W3033654687C1491633281 @default.
• W3033654687 hasConceptScore W3033654687C153074725 @default.
• W3033654687 hasConceptScore W3033654687C153911025 @default.
• W3033654687 hasConceptScore W3033654687C181080969 @default.
• W3033654687 hasConceptScore W3033654687C185592680 @default.
• W3033654687 hasConceptScore W3033654687C189165786 @default.
• W3033654687 hasConceptScore W3033654687C204787440 @default.
• W3033654687 hasConceptScore W3033654687C2776164576 @default.
• W3033654687 hasConceptScore W3033654687C2778155236 @default.
• W3033654687 hasConceptScore W3033654687C2779335624 @default.
• W3033654687 hasConceptScore W3033654687C2780550940 @default.
• W3033654687 hasConceptScore W3033654687C2780608848 @default.
• W3033654687 hasConceptScore W3033654687C2781403057 @default.
• W3033654687 hasConceptScore W3033654687C519063684 @default.
• W3033654687 hasConceptScore W3033654687C55493867 @default.
• W3033654687 hasConceptScore W3033654687C65001120 @default.
• W3033654687 hasConceptScore W3033654687C71924100 @default.
• W3033654687 hasConceptScore W3033654687C86803240 @default.
• W3033654687 hasConceptScore W3033654687C95444343 @default.
• W3033654687 hasIssue "Suppl 1" @default.
• W3033654687 hasLocation W30336546871 @default.
• W3033654687 hasOpenAccess W3033654687 @default.
• W3033654687 hasPrimaryLocation W30336546871 @default.
• W3033654687 hasRelatedWork W157476001 @default.
• W3033654687 hasRelatedWork W1586437180 @default.
• W3033654687 hasRelatedWork W1979409803 @default.
• W3033654687 hasRelatedWork W2034514523 @default.
• W3033654687 hasRelatedWork W2057691724 @default.
• W3033654687 hasRelatedWork W2065130305 @default.
• W3033654687 hasRelatedWork W2080548572 @default.
• W3033654687 hasRelatedWork W2183347089 @default.
• W3033654687 hasRelatedWork W2947427511 @default.
• W3033654687 hasRelatedWork W3033654687 @default.
• W3033654687 hasVolume "79" @default.
• W3033654687 isParatext "false" @default.
• W3033654687 isRetracted "false" @default.
• W3033654687 magId "3033654687" @default.
• W3033654687 workType "article" @default.