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• W3033735657 abstract "DNA has the potential to achieve a controllable macromolecular structure, such as hydrogels or droplets formed through liquid-liquid phase separation (LLPS), as the design of its base sequence can result in programmable interactions. Here, we constructed DNA droplets via LLPS of sequence-designed DNA nanostructures and controlled their dynamic functions by designing their sequences. Specifically, we were able to adjust the temperature required for the formation of DNA droplets by designing the sequences. In addition, the fusion, fission, and formation of Janus-shaped droplets were controlled by sequence design and enzymatic reactions. Furthermore, modifications of proteins with sequence-designed DNAs allowed for their capture into specific droplets. Overall, our results provide a platform for designing and controlling macromolecular droplets via the information encoded in component molecules and pave the way for various applications of sequence-designed DNA such as cell mimics, synthetic membraneless organelles, and artificial molecular systems." @default.
• W3033735657 created "2020-06-12" @default.
• W3033735657 creator A5003527421 @default.
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• W3033735657 date "2020-06-05" @default.
• W3033735657 modified "2023-10-16" @default.
• W3033735657 title "Sequence-based engineering of dynamic functions of micrometer-sized DNA droplets" @default.
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• W3033735657 doi "https://doi.org/10.1126/sciadv.aba3471" @default.
• W3033735657 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7269647" @default.
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